食品工业科技2024，Vol.45Issue(3)：277-283,7.DOI:10.13386/j.issn1002-0306.2023030304
Captiva EMR-Lipid技术结合UPLC-MS/MS快速测定牛羊产品中甲苯咪唑及其代谢物的残留量
Rapid Determination of Mebendazole and Its Metabolites in Cattle and Sheep Products by Captiva EMR-Lipid Technology Combined with UPLC-MS/MS
叶佳明 1钟世欢 1叶磊海 1吴余欣 2王京2
作者信息
- 1. 浙江公正检验中心有限公司,浙江杭州 310009||浙江省食品安全重点实验室,浙江杭州 310009
- 2. 浙江公正检验中心有限公司,浙江杭州 310009
- 折叠
摘要
Abstract
A method for detecting mebendazole and its metabolites in cattle and sheep products was established by EMR-Lipid-ultra-performance liquid chromatography-tandem mass spectrometry.The samples were first extracted by oscillation with 0.2%ammoniated acetonitrile solution,then freeze centrifugation,purified by Captiva EMR-Lipid column and directly analyzed by LC-MS/MS.The 3 kinds of mebendazole drugs were separated by SB-C18 column(2.1 mm×100 mm,1.8 μm)and gradiently eluted with acetonitrile and 0.1%formic acid water solution as mobile phase.The detection of mebendazole drugs was performed by tandem mass spectrometry in electrospray ionization under multiple reactions monitoring(MRM)mode.Matrix matching standard working curve and internal standard method were used for quantification.The calibration curves for target compounds were linear over the range of 0.2～50 ng/mL with correlation coefficients larger than 0.999.The detection limits(S/N>3)of 3 kinds of mebendazole drugs were 0.3 μg/kg.The recoveries of the 3 kinds of mebendazole drugs at three added levels were between 80.5%and 108.0%.The relative standard deviations were 1.1%～5.8%.The method overcame the current shortcomings of cumbersome preprocessing and long detection cycle,and it was simple in operation and accurate in results.The method was applicable to the rapid detection of mebendazole and its metabolites residues in cattle and sheep products.关键词
甲苯咪唑/氨基甲苯咪唑/羟基甲苯咪唑/液相色谱-串联质谱法/固相萃取Key words
mebendazole(MBZ)/hydroxy-mebendazole(MBZ-OH)/amino-mebendazole(MBZ-NH2)/liquid chromatography-tandem mass spectrometry(LC-MS/MS)/solid phase extraction(SPE)分类
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叶佳明,钟世欢,叶磊海,吴余欣,王京..Captiva EMR-Lipid技术结合UPLC-MS/MS快速测定牛羊产品中甲苯咪唑及其代谢物的残留量[J].食品工业科技,2024,45(3):277-283,7.
