食品工业科技2024，Vol.45Issue(3)：293-301,9.DOI:10.13386/j.issn1002-0306.2023040271
亲脂性匹配色谱分离-超高效液相色谱-串联质谱法测定果蔬中21种三唑类杀菌剂
Determination of 21 Triazole Fungicides in Fruits and Vegetables by Lipophilicity-matched Chromatographic Separation-Ultra Performance Liquid Chromatography-tandem Mass Spectrometry
摘要
Abstract
In order to improve the chromatographic separation efficiency of pesticide residues and reduce matrix effects,a lipophilicity-matched chromatographic separation strategy was proposed in this study.The triazole fungicides in fruits and vegetables were selected as a case study to establish the analytical method of 21 triazole fungicides with ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The samples were extracted by acetonitrile,followed by salting out and cleaned-up with dispersive solid phase extraction,then separated on chromatographic column with similar lipophilicity to triazole fungicides.The effects of alkyl bonded phase with different lipophilicity on the matrix effects,recoveries and chromatographic separation of triazole fungicides and matrix components were explored.The results showed that this strategy could improve the chromatographic separation of triazole fungicides and matrix components.And 21 triazole fungicides had matrix effects within-8.3%～4.7%and good linear relationship within 5～250 μg/L,the determination coefficient R2≥0.999.The average recoveries ranged from 91.4%to 108.1%,while the limits of quantitation were in the range of 0.5～3.5 μg/kg.This method could effectively reduce matrix effects and provide satisfactory recoveries using solvent calibration for quantification,which could significantly improve the detection efficiency.The proposed method was simple,accurate and sensitive,and suitable for determination of triazole fungicides in fruits and vegetables.This strategy could provide a method reference for column selection and matrix effect reduction in liquid chromatography-tandem mass spectrometry analysis.关键词
亲脂性匹配色谱分离/基质效应/三唑类杀菌剂/分散固相萃取/超高效液相色谱-串联质谱Key words
lipophilicity-matched chromatographic separation/matrix effect/triazole fungicides/dispersive solid-phase extraction/ultra performance liquid chromatography-tandem mass spectrometry分类
轻工纺织引用本文复制引用
孟虎,李源槿,赵丹妮,张雅婷,张丹,杨琦,冯歆轶..亲脂性匹配色谱分离-超高效液相色谱-串联质谱法测定果蔬中21种三唑类杀菌剂[J].食品工业科技,2024,45(3):293-301,9.基金项目
陕西省市场监督管理局科技计划项目(2020KY09). （2020KY09）
