食品工业科技2024，Vol.45Issue(3)：328-337,10.DOI:10.13386/j.issn1002-0306.2023030042
人参叶通过抑制细胞活性氧的积累延缓皮肤衰老
Ginseng Folium Attenuates Skin Aging by Limiting the Accumulation of Cellular Reactive Oxygen Species
摘要
Abstract
Objective:A network pharmacology-based approach,molecular docking and in vitro experimental validation were performed to reveal bioactive compounds and the molecular mechanism of ginseng folium via treating UVB-induced skin aging.Methods:The active components and targets of ginseng folium were obtained by TCMSP,and skin aging-related targets were retrieved by GeneCards and OMIM.Online Venn diagram was used to obtain the intersection targets.Cytoscape 3.7.0 software was used to conduct a comprehensive visual analysis of the multi-level network data of'drug-component-target-disease'.DAVID database was used to perform GO and KEGG enrichment analysis on the common targets.AutoDock docked the drugs to the predicted drug binding sites.The oxidative damage-related indicators and protein expression levels of HaCaT cells during UVB irradiation-induced senescence were detected in vitro.Results:As a result,four active components(sitosterol,quercetin,ginsenoside Rh2 and kaempferol)of ginseng folium and the 94 potential targets were predicted.The ginseng folium could significantly reduce the oxidative damage of the HaCaT aging model induced by UVB radiation,the same as the expression of p-JNK,and p-ERK proteins.Conclusion:Sitosterol,quercetin,ginsenoside Rh2,kaempferol,and other components in ginseng folium could activate the MAPK,PI3K/Akt,and other signal pathways through p-JNK,p-ERK,and other proteins to inhibit the production of lipid peroxides,protection on HaCaT cells,achieve multi-components,multi-targets,multi-channel effects,and resist the appearance and progress of skin aging.关键词
人参叶/皮肤衰老/UVB辐射/网络药理学/分子对接Key words
ginseng folium/skin aging/UVB-irradiation/network pharmacology/molecular docking分类
轻工纺织引用本文复制引用
张慧娥,杜连云,初孟瑶,宋欣宏,叶萍,王恩鹏..人参叶通过抑制细胞活性氧的积累延缓皮肤衰老[J].食品工业科技,2024,45(3):328-337,10.基金项目
吉林省科技发展计划项目(20210401108YY,20190304099YY) （20210401108YY,20190304099YY）
国家重点研发计划项目(2019YFC1710704) （2019YFC1710704）
珈伊生化研发中心(苏州工业园区)有限公司资助(20191120). （苏州工业园区）
