食品工业科技2024，Vol.45Issue(3)：338-345,8.DOI:10.13386/j.issn1002-0306.2023030326
鱼鳔与驴皮的化学成分及对环磷酰胺所致贫血小鼠的改善作用
Chemical Components of Swim Bladder and Donkey Skin and Their Improvement Effects on Cyclophosphamide-induced Anemia Mice
摘要
Abstract
Objective:To systematic study for different chemical components of swim bladder and donkey skin,and their improvement effects on cyclophosphamide-induced anemia mice.Methods:The chemical characterizations,including total protein content,amino acid composition and molecular weight were measured,respectively.Pepsin-soluble collagens were extracted from swim bladder and donkey skin by hydrochloric acid-pepsin method,and analyzed the content,composition and structure.The anemia model was established by intraperitoneal injected with cyclophosphamide at a dosage of 80 mg/kg.The coefficient of visceral organs and the hematological indexes were observed in mice with swim bladder glue(1.52 g/(kg·d))or donkey skin glue(1.39 g/(kg·d))by gavage administration per day.Results:The total protein contents of swim bladder and donkey skin reached more than 97%.The molecular weights of swim bladder glue and donkey skin glue ranged from 50 Da to 328 kDa.Swim bladder collagen and donkey skin collagen were both type Ⅰcollagen.While the content of collagen in swim bladder was 6.51 times than that in donkey skin.However the collagen triple helix structures were different.The swim bladder collagen had an α-helix structure,and the donkey skin collagen structure was mainly anti-parallel.After 28 days of administration,both swim bladder glue and donkey skin glue could promote the recovery of hematopoietic function in cyclophosphamide-induced anemia mice,and swim bladder glue exhibited a better effect.Compared with the model,the erythrocyte,hemoglobin,platelet,reticulocyte,spleen and thymus coefficients of the swim bladder glue were significantly improved(P<0.05).The erythrocyte,hemoglobin and thymus coefficients of the donkey skin glue were significantly improved(P<0.05).Conclusion:A systematic study of the chemical characteristics of swim bladder and donkey skin were carried out to determine the differences between the components.Pharmacodynamical research showed that swim bladder glue and donkey skin glue could promote the recovery of hematopoietic function in cyclophosphamide model mice,and the effect of swim bladder glue was better.It was hypothesized that the blood replenishing activity of the two might be related to the amino acids,collagen and other components.This work could provide a theoretical foundation for the development of swim bladder.关键词
鱼鳔/驴皮/化学成分/环磷酰胺/贫血/脏器系数/血液学指标Key words
swim bladder/donkey skin/chemical composition/cyclophosphamide/anemia/organ coefficient/hematology index分类
医药卫生引用本文复制引用
孙皓岩,胡淑曼,魏宝红,马晓青,杨文哲,刘佳,杨雪,邱培菊..鱼鳔与驴皮的化学成分及对环磷酰胺所致贫血小鼠的改善作用[J].食品工业科技,2024,45(3):338-345,8.基金项目
山东省重点研发计划(重大科技创新工程)(2021CXGC010510) （重大科技创新工程）
中央引导地方科技发展专项资金(YDZX2021018). （YDZX2021018）
