两阶段pH控制和碳氮源协同补加促进谷氨酸棒杆菌高产L-谷氨酰胺OACSTPCD
Two-stage pH control and synergistic addition of carbon and nitrogen sources for efficient L-glutamine production by Corynebacterium glutamicum
为提高谷氨酸棒杆菌发酵产L-谷氨酰胺产量、糖酸转化率、生产强度等技术指标,该文通过响应面法优化了摇瓶发酵培养基中葡萄糖、(NH4)2SO4和玉米浆含量,并利用50 L发酵罐进行分批发酵,构建了菌体生长、底物消耗和产物生成动力学模型,进而在50 L发酵罐中采用两阶段控制pH策略,并通过补加葡萄糖和(NH4)2SO4,协同控制发酵液中碳氮源浓度,促进菌体高效合成L-谷氨酰胺.结果表明,优化后发酵培养基中葡萄糖、(NH4)2SO4和玉米浆含量分别为16…查看全部>>
To improve the fermentation level,sugar-acid conversion rate,and productivity of L-glutamine production by Corynebacte-rium glutamicum,the contents of glucose,ammonium sulfate,and corn syrup in fermentation medium were optimized using response sur-face methodology.Then the kinetic models for cell growth,substrate consumption and L-glutamine production of batch fermentation in 50 L fermenter were constructed.Furthermore,the strategy of two-stage pH control co…查看全部>>
刘畅;陆丹丹;浦军平;张春枝;陈明
大连工业大学生物工程学院,辽宁大连,116034张家港市华昌药业有限公司,江苏苏州,215635张家港市华昌药业有限公司,江苏苏州,215635大连工业大学生物工程学院,辽宁大连,116034大连工业大学生物工程学院,辽宁大连,116034
谷氨酸棒杆菌L-谷氨酰胺培养基优化50 L发酵罐两阶段pH控制碳氮源协同补加
Corynebacterium glutamicumL-glutaminemedium optimization50 L fermentertwo-stage pH controlsynergistic addition of carbon and nitrogen sources
《食品与发酵工业》 2024 (1)
7-13,7
辽宁省高等学校创新人才支持计划项目(LR201907)
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