一株高效降解马铃薯糖苷生物碱菌株的筛选鉴定及降解特性研究OACSTPCD

Improper storage of potatoes produces toxic glycoalkaloids(GAs),posing a severe threat to human health.Biodegradation is the first-choice technology to effectively degrade GAs from potato.In this study,endophytic bacteria were isolated from sprouted and green potato tubers.The inorganic salt medium with GAs as the sole carbon source was used for screening GA degrading bacteria.The strains were identified by physiological and biochemical determination and 16S rDNA sequencing.Then the safety of GA degrading bacteri-a was studied.Finally,the degradation characteristics and products of GAs were detected by HPLC and liquid chromatography-ion trap-time of flight mass spectrometry(LC-IT-TOF-MS).The results showed that strain C11,which could efficiently degrade GAs,was screened and identified as Bacillus velezensis.The strain C11 was resistant to some antibiotics but did not contain plasmid,did not produce harmful metabolites,and had no hemolytic activity.So,strain C11 had certain safety.The optimum degradation conditions of the strain C11 were 30 ℃ and pH 6.0.Mn2+could increase the degradation ability,while Cu2+could strongly inhibit the degradation.Additional-ly,strain C11 may degrade GAs into cholesterol and further metabolize and utilize them in the cell.The strain C11 has high efficiency of GAs degradation and excellent environmental adaptability,which has a high application prospect in the development of bacterial agents.