牡蛎提取物对秀丽隐杆线虫的抗衰老作用研究OACSTPCD

To compare the anti-aging effects of two oyster enzymatic extracts,including enzymolysis extract of oyster(EPO),oyster peptide(OP),and oyster water extract(WPO),the basic components of the three oyster extracts were determined,and Caenorhabditis elegans was used as an animal model to study the effects of oyster extracts on lifespan,reproduction,lipofuscin accumulation,athletic ability,acute oxidative stress injury,and redox level in the body.Results showed that the basic components of EPO,OP,and WPO were different,but the main components were all proteins.Among them,OP had the highest total protein content with(66.59±0.57)g/100 g dry basis.Compared with control,the low,medium,and high concentrations(50,200,800 μg/mL)of EPO,OP,and WPO prolonged C.elegans lifespan,but the effect was not significant at low concentrations.Between three oyster extracts,only OP significantly increased the egg production of C.elegans in a concentration-dependent manner(P＜0.05),with a maximum increase of 80.2％.All three ex-tracts significantly reduced the lipofuscin accumulation in C.elegans(P＜0.05)in a dose-dependent manner with a maximum of 98.15％.High concentrations of three oyster extracts(800 μg/mL)significantly improved the movement ability of aging C.elegans,in-creased the survival rate of C.elegans damaged by paraquat(P＜0.05)as well as the activity of GSH-Ps and SOD enzymes,and reduced MDA contents in vivo by 89.2％to 98.2％(P＜0.05).The effects of three oyster extracts had the order of OP＞EPO＞WPO.In con-clusion,EPO,OP,and WPO showed different anti-aging effects.OP showed the best effect,which could provide a reference for further development of oyster foods for anti-aging.