基于表型性状构建禾初级核心种质OACSTPCD

'HE'is a rice landraces that adapts to the special natural habitat of shading,slough,cold and waterlogged in deep-valley and forests in the Qiandongnan mountainous areas in Guizhou.In this study,402 HE resources,divided into 5 groups based on the color differences of the glume tip,were used as materials.Based on the 26 phenotypic traits,the progressive clustering method was applied to screen out the optional sampling strategy by using 6 overall sampling scales,4 intra-group sampling ratios and 2 intra-group sampling methods,and to construct the primary core collection and evaluate it comprehensively.The results showed that 10％was the optimal overall sampling scale,the optimal intra-group sampling ratio method was the logarithmic ratio method,and the best intra-group sampling method was the cluster sampling method.Under the optimal sampling strategy,47 primary core collections of HE was obtained,accounting for 11.69％of the whole germplasm,and the retention rate of phenotype was 95.77％.The core collection constructed in this study could be a good representation of the genetic diversity of the original germplasm as a concentration of it.