血清SOX6、VCAN表达对胃癌诊断及病情评估的价值分析OACSTPCD
Value of the expression of serum SOX6 and VCAN in the diagnosis and condition evaluation of gastric cancer
目的 探讨胃癌患者血清中性别决定区Y框蛋白6(SOX6)、多功能蛋白聚糖(VCAN)的表达情况,并分析其对胃癌患者诊断及病情评估的价值.方法 选取2019年1月至2021年10月本院收治的胃癌患者105例为胃癌组,依据病理分期将患者分为Ⅰ、Ⅱ期组(37例)和Ⅲ、Ⅳ期组(68例);另选取本院收治的胃良性病变患者85例为良性组;同时选取85例健康体检者为对照组.采用实时qRT-PCR法对血清中SOX6、VCAN mRNA表达水平进行检测,采用Spearman法分析SOX6、VCAN表达与病理分期的相关性;受试者工作特征(ROC)曲线评价血清SOX6、VCAN表达对胃癌的诊断价值.结果 胃癌组患者血清中SOX6表达水平低于对照组和良性组,VCAN表达水平高于对照组和良性组,差异均有统计学意义(P<0.05).胃癌Ⅲ、Ⅳ期组患者血清中VCAN表达水平高于Ⅰ、Ⅱ期组患者,SOX6表达水平低于Ⅰ、Ⅱ期组患者,差异均有统计学意义(P<0.05).相关性分析结果显示,胃癌患者血清中SOX6表达与病理分期呈负相关(r=-0.485,P<0.05),VCAN表达与病理分期呈正相关(r=0.518,P<0.05).ROC曲线结果显示,SOX6、VCAN预测胃癌患者的曲线下面积(AUC)分别为0.921(95%CI:0.873～0.955)、0.915(95%CI:0.865～0.950),敏感度分别为92.38%、79.05%,特异度分别为 83.53%、95.29%;二者联合诊断胃癌患者的AUC为 0.969(95%CI:0.934～0.989),敏感度为94.29%,特异度为90.59%.结论 胃癌患者血清中SOX6表达水平降低,VCAN表达水平升高,二者联合检测可作为胃癌诊断的血清标志物,同时可间接预测胃癌病情程度.
Objective To investigate the expression of serum sex determining region Y-box 6(SOX6)and versican(VCAN)in patients with gastric cancer,and to analyze their value in the diagnosis and condition evaluation of patients with gastric cancer.Methods A total of 105 patients with gastric cancer admitted to our hospital from January 2019 to October 2021 were selected as the gastric cancer group,and they were divided into stage Ⅰ and Ⅱ group(37 cases)and stage Ⅲ and Ⅳ group(68 cases)according to pathological stages;85 patients with benign gastric lesions admitted to our hospital were selected as the benign group,and 85 healthy people were selected as the control group.The expression levels of serum SOX6 and VCAN mRNA were detected by qRT-PCR,the correlation between SOX6 and VCAN expression and pathological stage was analyzed by Spearman method.Receiver operating characteristic(ROC)curve was used to evaluate the diagnostic value of serum SOX6 and VCAN expression for gastric cancer.Results The expression level of serum SOX6 in gastric cancer group was lower than those in the control group and the benign group,and the expression level of VCAN was higher than those in the control group and the benign group,with statistical significance(P<0.05).The expression level of serum VCAN of patients with gastric cancer in stage Ⅲand Ⅳ group was higher than that in stage Ⅰ and Ⅱ group,and the expression level of SOX6 was lower than that in stage Ⅰ and Ⅱ group,with statistical significance(P<0.05).The results of correlation analysis showed that the expression of serum SOX6 in patients with gastric cancer was negatively correlated with pathological stage(r=-0.485,P<0.05),and the expression of VCAN was positively correlated with the pathological stage(r=0.518,P<0.05).The results of ROC curve showed that the area under the curve(AUC)of SOX6 and VCAN in predicting patients with gastric cancer were 0.921(95%CI:0.873 to 0.955)and 0.915(95%CI:0.865 to 0.950),respectively,the sensitivity were 92.38%and 79.05%,respectively,the specificity were 83.53%and 95.29%,respectively;the AUC of the combined diagnosis of SOX6 and VCAN for patients with gastric cancer was 0.969(95%CI:0.934 to 0.989),the sensitivity was 94.29%,and the specificity was 90.59%.Conclusion The expression level of serum SOX6 in patients with gastric cancer is decreased,and the expression level of VCAN is increased.The combined detection of SOX6 and VCAN can be used as serum markers for the diagnosis of gastric cancer,and can indirectly predict the condition severity of gastric cancer.
黄小平;李轶;李萍
西安市第九医院消化内科,陕西 西安 710054西安市第九医院血管介入科,陕西 西安 710054
临床医学
胃癌性别决定区Y框蛋白6多功能蛋白聚糖相关性
gastric cancersex determining region Y-box 6versicancorrelation
《局解手术学杂志》 2024 (002)
149-152 / 4
西安市第九医院科研项目(2020-7)
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