中国食品添加剂2024，Vol.35Issue(1)：71-76,6.DOI:10.19804/j.issn1006-2513.2024.1.009
弱电场中油菜籽蛋白提取优化及结构测定
Optimization of rapeseed protein extraction in weak electric field and rapeseed structure analysis
摘要
Abstract
Using cold-pressed rapeseed as raw material,the weak electric field was introduced in the extraction of rapeseed protein.The single factor and orthogonal experiments were used to optimize the extraction conditions.The results showed that the order of factors affecting the extraction rate of rapeseed protein in weak electric field was voltage＞temperature＞material liquid ratio.The optimal extraction process was as follow:electric field voltage 15 V,extraction temperature 30℃,and material liquid ratio 1∶10(g/mL).Under the above conditions,the extraction ratio of rapeseed protein could reach 7.59%.The protein structure was analyzed by infrared spectrum and element analysis.The results showed that three obvious amide Ⅰ,Ⅱ,and Ⅲ bands in the extracted product,with the purity of protein was 88.75%.Finally,scanning electron microscope showed that the extracted protein was irregular granules.The results demonstrated high purity proteins could be obtained by using electric field flocculation.The study provides theoretical support for extraction of plant proteins in weak electric field.关键词
油菜籽粕/油菜籽蛋白/弱电场/正交试验/蛋白质絮凝Key words
rapeseed meal/rapeseed protein/weak electric field/orthogonal test/flocculation of protein分类
轻工纺织引用本文复制引用
戴新赫,贺颖,赵立,蒋舒婷,仇丹,王亚娟..弱电场中油菜籽蛋白提取优化及结构测定[J].中国食品添加剂,2024,35(1):71-76,6.基金项目
国家青年基金科学基金项目(51703100) （51703100）
宁波市自然科学基金(2021J142) （2021J142）
校级创新项目(2021034). （2021034）
