中国食品添加剂2024，Vol.35Issue(1)：143-149,7.DOI:10.19804/j.issn1006-2513.2024.1.017
响应面法优化超声辅助提取姬菇多糖及其体外抗氧化性
Optimization of ultrasound-assisted extraction of polysaccharides from Agaricus blazei by response surface methodology and its in vitro antioxidant activity
摘要
Abstract
Polysaccharides in Agaricus blazei were extracted by ultrasonic assisted method.Using the extraction rate as an indicator,the effects of liquid to material ratio,extraction temperature,extraction time,and ultrasonic power on the extraction rate of polysaccharides from Agaricus blazei were firstly investigated through single factor experiment.Then,response surface methodology was designed to optimize the parameters of extraction process of Agaricus blazei polysaccharides.The results showed that the highest extraction rate of polysaccharides from Agaricus blazei could reach 16.38%,when the conditions of extraction process were as follows:material to liquid ratio of 30 g/g,extraction temperature of 75℃,ultrasonic assisted power of 500 W,extraction time of 80 minutes.After extraction,the crude polysaccharide of Agaricus blazei was deproteinized by TCA,decolorized by hydrogen peroxide and dialyzed to obtain purified refined polysaccharide.Crude polysaccharide and refined polysaccharide had certain ability to scavenge hydroxyl and ABTS+ free radicals,the antioxidant activity of refined polysaccharide was lower than that of crude polysaccharide,and the antioxidant activity of both crude and refined polysaccharides were lower than that of VC.Therefore,the purity of Agaricus blazei polysaccharides had a significant impact on its antioxidant activity,the higher the purity,the lower the antioxidant activity.关键词
姬菇/多糖/响应面/抗氧化Key words
Agaricus blazei/polysaccharide/response surface/antioxidation分类
轻工纺织引用本文复制引用
陈蕾,郑甜甜,朱贺宇,王卫东,孙月娥,宫号..响应面法优化超声辅助提取姬菇多糖及其体外抗氧化性[J].中国食品添加剂,2024,35(1):143-149,7.基金项目
江苏省政策引导类计划项目(XZ-SZ202148,XZ-SZ202156) （XZ-SZ202148,XZ-SZ202156）
徐州市科技计划重点研发计划(KC22082). （KC22082）
