中国食品添加剂2024，Vol.35Issue(1)：162-170,9.DOI:10.19804/j.issn1006-2513.2024.1.019
辽东楤木芽多糖乙酰化修饰工艺及其抗氧化活性研究
Optimization of acetylated modification of polysaccharide extracted from Aralia elata(Miq.)Seem.bud and its antioxidant activity
摘要
Abstract
Using Aralia elata(Miq.)Seem.bud(AEBP)as raw material,Polysaccharides from AEBP was extracted by ultrasonic assisted extraction,and acetylation of polysaccharides from Aralia elata(Miq.)Seem.bud(Ac-AEBP)was prepared.The degree of acetylation substitution was used as index,the effects of reaction temperature,reaction time and liquid-material ratio(mL/g)on AEBP acetylation modification substitution were studied.On the basis of single factor experiment,parameters of the acetylation process were optimized using Box-Behnken design response surface experiment.The scavenging effect and reducing ability of AEBP and Ac-AEBP on·OH,·O2-DPPH·and ABTS+·were compared to study their antioxidant activities.The optimal preparation conditions of Ac-AEBP were as follows:reaction temperature 52℃,reaction time 4 h and liquid-material ratio 43∶1(mL/g).Under the optimized conditions,the degree of Ac-AEBP substitution was 0.4596.Antioxidant results showed that,compared with unmodified AEBP,Ac-AEBP antioxidant capacity were greatly enhanced.The study provides a scientific basis for the further development and utilization of Aralia elata(Miq.)Seem.bud.关键词
辽东楤木芽多糖/乙酰化修饰/响应面/抗氧化活性Key words
Aralia elata(Miq.)Seem.bud polysaccharide/acetylation modification/response surface/antioxidant activity分类
轻工纺织引用本文复制引用
宫益霞,孔凡星,卢玉婷,任杰,谢炜康,王丽红..辽东楤木芽多糖乙酰化修饰工艺及其抗氧化活性研究[J].中国食品添加剂,2024,35(1):162-170,9.基金项目
黑龙江省自然科学基金项目(YQ2021H027) （YQ2021H027）
黑龙江省北药与功能食品特色学科项目(No.HLJTSXK-2022-03) （No.HLJTSXK-2022-03）
佳木斯大学国家培育项目(JMSUGPZR2207) （JMSUGPZR2207）
针对防治常见慢性病的植物药开发利用研究创新团队(cxtd202103). （cxtd202103）
