中国食品学报2023，Vol.23Issue(12)：77-86,10.DOI:10.16429/j.1009-7848.2023.12.009
NaCl对可口革囊星虫体壁肌原纤维蛋白理化和凝胶性质的影响
Effects of NaCl on Physicochemical and Gel Properties of Myofibrillar Protein from the Body Wall of Phascolosoma esculenta
摘要
Abstract
In order to investigate the effects of NaCl on the physicochemical and gel properties of myofibrillar protein(PeMP)from the body wall of Phascolosoma esculenta Chen et.Yeh,the determination and observation results of solu-bility of total PeMP,solubility of different types of proteins from PeMP,ultraviolet absorption spectrum,endogenous flu-orescence spectrum,Zeta potential,Fourier transform infrared spectrum(FTIR),sulfhydryl group contents,surface hy-drophobicity,denaturation temperature and gel structure were analyzed.The results showed that the solubility of PeMP increased,the contents of paramyosin and myosin increased significantly,with the increase of NaCl concentration.The maximum absorption wavelength(λmax)of the ultraviolet absorption spectrum of PeMP,of the corresponding second derivative spectrum of ultraviolet absorption spectrum of PeMP and of the endogenous fluorescence spectrum of PeMP was redshifted,respectively.The Zeta potential of PeMP increased.FTIR analysis showed that the secondary structure of PeMP changed,the β-sheet increased,while the α-helix decreased gradually.The contents of total and non-protein sulfhydryl groups decreased in PeMP,but the contents of typing sulfhydryl group increased,and the surface hydropho-bicity of PeMP increased.The denaturation temperature of PeMP decreased.Protein molecules of PeMP gel were more tightly packed.These results will lay a foundation for the improvement of the gel of body wall from P.esculenta and the preparation of high-quality'earth bamboo shoot jelly'products.关键词
可口革囊星虫/肌原纤维蛋白/理化性质/凝胶性质/氯化钠Key words
Phascolosoma esculenta Chen et.Yeh/myofibrillar protein(PeMP)/physicochemical property/gel property/NaCl引用本文复制引用
方佳琪,薛茜,王芳,陈朝阳,蔡荣杰,郭凤仙,汤璧蔚,黄慧..NaCl对可口革囊星虫体壁肌原纤维蛋白理化和凝胶性质的影响[J].中国食品学报,2023,23(12):77-86,10.基金项目
2021年国家大学生创新创业训练计划项目(202110399014) （202110399014）
