中国食品学报2023，Vol.23Issue(12)：167-178,12.DOI:10.16429/j.1009-7848.2023.12.018
花生红衣原花青素纯化工艺及其对乳糜泻细胞模型的影响
Purification Technology of Peanut Red Procyanidins and Its Effect on Celiac Disease Cell Model
摘要
Abstract
Objective:To optimize the purification process of AB-8 macroporous resin for peanut red procyanidins and to characterize and identify the components of the purified product,and to evaluate its inhibitory effect on cytotoxicity in celiac disease model.Methods:AB-8 macroporous resin was selected,and the purification conditions were optimized by static and dynamic adsorption-desorption indexes.The purified material was analyzed and identified by Ultraviolet/visible spectroscopy,Fourier transform infrared spectroscopy and High performance liquid chromatography-tandem mass spectrom-etry(UPLC-QTOF-MS/MS).The inhibitory effect of purified material on cytotoxicity was evaluated based on celiac disease cell model.Results:Purification conditions:The adsorption time was 180 min.The desorption solution was 120 mL 40％ethanol solution.The desorption time was 40 min.The desorption flow rate was 1.0 mg/mL.Ultraviolet/visible spectrum analysis showed that the purified substance belonged to proanthocyanidins.Fourier infrared analysis showed that proto-cyanin was the main structural unit of the purified product,which was consistent with the characteristic absorption peak trend of the standard product.UPLC-QTOF-MS/MS analysis showed that type A procyanidins were the main component of Pspc purified products.The results of cell test showed that the purified substance could significantly inhibit the toxicity of celiac disease model to some extent.This study can provide some theoretical reference for comprehensive development of peanut red resources.关键词
花生红衣原花青素/AB-8大孔树脂/高效液相色谱串联质谱(UPLC-QTOF-MS/MS)/细胞毒性Key words
peanut red procyanidins/Ab-8 macroporous resin/high performance liquid chromatography tandem mass spectrometry(UPLC-QTOF-MS/MS)/cytotoxicity引用本文复制引用
王娜,郑玉茹,徐超,李军伟,任红涛,余秋颖..花生红衣原花青素纯化工艺及其对乳糜泻细胞模型的影响[J].中国食品学报,2023,23(12):167-178,12.基金项目
河南省创新生态支撑专项(HARS-22-05-Z1) （HARS-22-05-Z1）
