中国食品学报2023，Vol.23Issue(12)：238-246,9.DOI:10.16429/j.1009-7848.2023.12.025
石斛属植物DNA条形码鉴定及铁皮石斛产地分析
DNA Barcoding Identification of Dendrobium and Analysis of Dendrobium officinale Habitats
摘要
Abstract
To identify Dendrobium species and to analyze the habitats of Dendrobium officinale,the suitable candidate DNA barcoding genes of Dendrobium were evaluated.In this study,the internal transcribed spacer 2(ITS2)of nuclear ribosomal DNA and chloroplast psbA-trnH gene were amplified and sequenced from sixty-six samples of D.chrysotoxum,D.nobile,D.primulinum and D.officinale.The D.officinale samples were collected from Hangzhou,Huoshan,Yunnan and Wenzhou.The intra-specific and inter-specific divergence of Dendrobium was calculated,and the identification efficiency was assessed using Barcoding Gap,NJ tree by K2P distance.The results showed the intra-specific divergence of ITS2(0-0.005)were obviously lower than the inter-specific divergence(0.070-0.228),and four species of Dendrobium were also accurately distinguished in NJ tree.Whereas,there is no Barcoding Gap on psbA-trnH,and it cannot distinguish D.nobile and D.primulinum in the NJ tree,and it can be used as a supplementary sequence in the identification of Dendrobium.ITS2 and psbA-trnH of D.officinale from four habitats have multiple mutation sites,which have the potential to identify the habitats of D.officinale.关键词
石斛属/铁皮石斛/DNA条形码/鉴定Key words
Dendrobium/Dendrobium officinale/DNA barcoding/identification引用本文复制引用
鄢珊珊,沈斌国,赵海莹,丁艳菲,朱诚..石斛属植物DNA条形码鉴定及铁皮石斛产地分析[J].中国食品学报,2023,23(12):238-246,9.基金项目
台州市"500精英计划"人才项目(B类) （B类）
浙江省自然科学基金项目(LZ22C130003) （LZ22C130003）
浙江省科技计划项目(2022C04002) （2022C04002）
浙江省高校基本科研业务费项目(2022YW79) （2022YW79）
