中国食品学报2023,Vol.23Issue(12):302-310,9.DOI:10.16429/j.1009-7848.2023.12.031
灰树花水上清液中不同极性提取物的功能成分及其抗氧化活性
Functional Components and Antioxidant Activities of Different Polarities Extracts from the Supernatant of Grifola frondosa
摘要
Abstract
In the study,petroleum ether,ethyl acetate,n-butanol and water with different polar solvents were used to the water-extracted supernatant of Grifola frondosa.The main functional components of different polar extracts were com-pared,and the antioxidant activities were compared by three methods:1,1-Diphenyl-2-trinitrophenylhydrazine(DPPH),2,2-diazo-bis(3-ethyl-benzothiazole-6-sulfonic acid)diammonium salt(ABTS+),hydroxyl(OH)free radical.The results showed that the polyphenol and protein contents of the n-butanol extract were the highest at 0.36%and 38.75%,re-spectively.The highest flavonoid content in petroleum ether and ethyl acetate extracts was 0.05%.The contents of triter-penoids and sterols in petroleum ether extract were 6.32%and 15.88%,respectively.The highest polysaccharide content in water extract was 30.11%.N-butanol extract showed the strongest antioxidant activity,and polyphenols may be the main component of antioxidant activity.Thirteen polyphenols and their derivatives were identified by LC-MS.This pro-vides a theoretical basis for the development of Grifola frondosa functional food or health products.关键词
灰树花/水提上清液/功能成分/抗氧化Key words
Grifola frondosa/water-extracted supernatant/functional components/antioxidant引用本文复制引用
熊雯宇,何君强,刘斌..灰树花水上清液中不同极性提取物的功能成分及其抗氧化活性[J].中国食品学报,2023,23(12):302-310,9.基金项目
福建省科学技术厅对外合作项目(202110008) (202110008)