中国食物与营养2023，Vol.29Issue(12)：42-47,6.
辣木叶水提物成分分析及其对小鼠力竭游泳时间的影响
Constituent Analysis of Moringa Oleifera Leaves Aqueous Extract and Its Effect on Exhaustive Swimming Time in Mice
董维雲 1边祥雨 1杨忍忍 1金璐 1井澈 1高蔚娜 1郭长江1
作者信息
- 1. 军事科学院军事医学研究院环境医学与作业医学研究所,天津 300050
- 折叠
摘要
Abstract
[Objective]To investigate the active constituents of Moringa oleifera leaves aqueous extract,and to evaluate its effect on exhaustive swimming time in mice.[Method]The aqueous extract of Moringa oleifera Leaves was prepared by ultrasound-assisted aqueous extraction.Total polyphenols,total flavonoids and total polysaccharides were assayed by folin-ciocalteau method,aluminate chromogenic method,and phenol-vitriolic colorimetry method,respectively.The antioxidant activities were measured by FRAP method and commercial kits.The polyphenols were analyzed by UPLC-MS/MS.The effect of the aqueous extract on exhaustive swimming time in mice was obser-ved.[Result]The average contents of total polyphenols,flavonoids and polysaccharides were(34.6±3.8)mg GAE/g,(39.9±2.0)mg RE/g,(239.6±12.4)mg AE/g,respectively.The total antioxidant capacity,and clearance rates of hydroxy radical and DPPH in vitro were(285.7±13.9)U/g,(27.7±5.7)％and(56.6±3.4)％,respectively.UPLC-MS/MS analysis showed that the relatively contents of luteo-lin,quercetin,kaempferol and chlorogenic in Moringa oleifera leaves aqueous extract were high.Compared with the control group,the aqu-eous extract significantly improved the exhaustive swimming time of mice(P＜0.05).[Conclusion]The Moringa oleifera leaves aqueous extract is rich in polyphenols,flavonoids and polysaccharides,possesses antioxidant capacity,and has anti-fatigue effect to some extent.关键词
辣木叶/水提取物/成分分析/力竭游泳时间Key words
Moringa oleifera leaf/aqueous extract/constituent analysis/exhaustive swimming time引用本文复制引用
董维雲,边祥雨,杨忍忍,金璐,井澈,高蔚娜,郭长江..辣木叶水提物成分分析及其对小鼠力竭游泳时间的影响[J].中国食物与营养,2023,29(12):42-47,6.
