水貂阿留申病毒核酸标准物质的研制OACSTPCD

In order to prepare a uniform and stable nucleic acid reference material for Aleutian mink disease virus(AMDV),AMDV-G strain was selected and inoculated into CRFK cells for virus reproduc-tion,after inactivation,the nucleic acid reference material was successfully prepared.We used the droplet digital PCR method to conduct homogeneity and stability,and collaborative calibration with 8 laboratories on the prepared nucleic acid reference materials.The homogeneity test showed that F=1.56＜Fa(F(0.05,14,30)=2.04),indicated that there was no statistical difference within or between groups of this nucleic acid reference materials.The stability evaluation results indicated that,this nucleic acid reference materials can be stable for 6 months at-20 ℃,9 days at 4 ℃,and 4 freeze-thaw cycles at-20℃.Collaborated with 8 laboratories with laboratory testing qualifications for fixed value and uncertainty assessment,the quantity value was 1.05 × 104copies/μL,the expanded uncertainty was 0.14 × 104 copies/μL.In summary,the prepared nucleic acid reference materials were advantaged by accurate calibration,great homogeneity and stability evaluation,it can be used for related experi-ments such as AMDV nucleic acid detection,which is beneficial for the diagnosis,prevention and control of Aleutian mink disease.