中国兽医科学2024，Vol.54Issue(1)：26-33,8.DOI:10.16656/j.issn.1673-4696.2024.0006
水貂阿留申病毒核酸标准物质的研制
Preparation of nucleic acid reference materials of Aleutian mink disease virus
摘要
Abstract
In order to prepare a uniform and stable nucleic acid reference material for Aleutian mink disease virus(AMDV),AMDV-G strain was selected and inoculated into CRFK cells for virus reproduc-tion,after inactivation,the nucleic acid reference material was successfully prepared.We used the droplet digital PCR method to conduct homogeneity and stability,and collaborative calibration with 8 laboratories on the prepared nucleic acid reference materials.The homogeneity test showed that F=1.56＜Fa(F(0.05,14,30)=2.04),indicated that there was no statistical difference within or between groups of this nucleic acid reference materials.The stability evaluation results indicated that,this nucleic acid reference materials can be stable for 6 months at-20 ℃,9 days at 4 ℃,and 4 freeze-thaw cycles at-20℃.Collaborated with 8 laboratories with laboratory testing qualifications for fixed value and uncertainty assessment,the quantity value was 1.05 × 104copies/μL,the expanded uncertainty was 0.14 × 104 copies/μL.In summary,the prepared nucleic acid reference materials were advantaged by accurate calibration,great homogeneity and stability evaluation,it can be used for related experi-ments such as AMDV nucleic acid detection,which is beneficial for the diagnosis,prevention and control of Aleutian mink disease.关键词
水貂阿留申病毒/核酸标准物质/微滴式数字PCRKey words
Aleutian mink disease virus/nucleic acid reference material/droplet digital PCR分类
农业科技引用本文复制引用
王春霞,高华,程悦宁,王振军,商金源,吴顺,闫曼平,冯二凯,罗国良,易立,叶京飞,赵宗正..水貂阿留申病毒核酸标准物质的研制[J].中国兽医科学,2024,54(1):26-33,8.基金项目
中国农业科学院科技创新工程项目(CAAS-ASTIP-2021-ISAPS) （CAAS-ASTIP-2021-ISAPS）
吉林省科技创新平台项目(YDZJ202302CXJD034) （YDZJ202302CXJD034）
吉林省科技发展计划项目(20210202099NC) （20210202099NC）
吉林省发改委项目(2022C042-9) （2022C042-9）
