中国畜牧兽医2024，Vol.51Issue(1)：347-356,10.DOI:10.16431/j.cnki.1671-7236.2024.01.035
1株羊源多杀性巴氏杆菌的分离鉴定及病原特性研究
Isolation,Identification and Pathogenic Characteristics of One Pasteurella multocida Isolate from Sheep
摘要
Abstract
[Objective]This study was aimed to identify the pathogenic bacteria that causing the disease and death of breeding sheep in a sheep farm in Qiqihar city,and to explore its etiological characteristics,so as to provide scientific basis for effective prevention and control of the disease.[Method]The lungs from dead sheep were aseptically taken,and laboratory diagnosis was conducted based on clinical symptoms.Pathogenic bacteria were isolated and cultured.Morphological observation,biochemical test,16S rRNA PCR amplification and sequencing,kmt Ⅰgene identification,capsule serotyping,virulence gene detection,drug susceptibility test,drug resistance gene detection and mouse pathogenicity test were used to identify and analyze the pathogenic characteristics of the isolated bacteria.[Result]The pathogenic strain was Pasteurella multocida.The isolated bacteria showed round raised colonies with smooth,grayish white surface on the blood plate,and Gram staining showed that the Gram-negative brevibacterium was clustered into lines or scattered in single.Biochemical test results showed that the strain could ferment maltose,arabinose,sucrose,xylose,glucose,fructose,trehalose,mannitol,urease,sorbitol and and ONPG,and arginine acid hydrolysis and indigo test were positive.Serotyping of the capsule showed that the isolate was Pasteurella multocida capsular serotype D.The drug sensitivity test showed that the isolates were resistant to enrofloxacin,bacitracin and lomefloxacin,intermediary to 4 drugs such as neomycin,and sensitive to 16 drugs such as ampicillin.The isolates carried 14 virulence genes and 6 resistance genes.All mice died after infection with the isolated bacteria.[Conclusion]One Pasteurella multocida serotype D strain was successfully isolated,carrying a variety of virulence genes and drug resistance genes,and was pathogenic to mice.This study provided data for exploring the biological information and etiological characteristics of the sheep origin Pasteurella multocida.关键词
多杀性巴氏杆菌/分离鉴定/毒力基因/耐药性/致病性Key words
Pasteurella multocida/isolation and identification/virulence gene/drug resistance/pathogenicity分类
农业科技引用本文复制引用
张蕾,张备,董佳强,黄宣凯,李旭业,江波涛,王洪宝,史同瑞,陈亮,金振华,冯万宇,苗艳,兰世捷,秦平伟,沈思思,李丹..1株羊源多杀性巴氏杆菌的分离鉴定及病原特性研究[J].中国畜牧兽医,2024,51(1):347-356,10.基金项目
黑龙江省农业科学院畜牧兽医分院自拟课题(ZNKT-202212) （ZNKT-202212）
齐齐哈尔市科技计划创新激励项目(CNYGG-2023019) （CNYGG-2023019）
黑龙江省农业科技创新跨越工程农业关键技术科技创新重点攻关项目"草食家畜新品种选育推广与产业应用"(CX23GG07) （CX23GG07）
