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1株羊源多杀性巴氏杆菌的分离鉴定及病原特性研究OACSTPCD

Isolation,Identification and Pathogenic Characteristics of One Pasteurella multocida Isolate from Sheep

中文摘要英文摘要

[目的]确定齐齐哈尔市某羊场育成绵羊发病和死亡的病原菌,并探究其病原学特性,为该病的有效防治提供科学依据.[方法]无菌采集病死羊肺脏组织,结合临床病征进行实验室诊断,分离培养病原菌.通过形态学观察、生化试验、16S rRNA PCR扩增并测序、kmt Ⅰ基因鉴定、荚膜血清分型、毒力基因检测、药敏试验、耐药基因检测和小鼠致病性试验对分离菌进行鉴定和病原特性分析.[结果]致病菌株为多杀性巴氏杆菌,分离菌在血平板上长出表面光滑、灰白色的圆形凸起菌落,革兰染色可见聚集成线状或散在单个的革兰阴性短杆菌.生化试验结果显示,该菌能发酵麦芽糖、阿拉伯糖、蔗糖、木糖、葡萄糖、果糖、海藻糖、甘露醇、尿素酶、山梨醇和β-半乳糖苷酶,精氨酸、靛基质试验呈阳性.荚膜血清分型结果显示,分离株为多杀性巴氏杆菌荚膜D型.药敏试验结果显示,分离株对恩诺沙星、杆菌肽和洛美沙星3种药物耐药,对新霉素等4种药物表现为中介,对氨苄西林等16种药物敏感.分离株携带14种毒力基因和6种耐药基因.小鼠感染分离菌后全部死亡.[结论]本研究成功分离到1株D型多杀性巴氏杆菌菌株,其携带多种毒力基因和耐药基因,对小鼠具有较强致病性,研究结果为探究羊源多杀性巴氏杆菌的生物学信息和病原学特征提供了数据.

[Objective]This study was aimed to identify the pathogenic bacteria that causing the disease and death of breeding sheep in a sheep farm in Qiqihar city,and to explore its etiological characteristics,so as to provide scientific basis for effective prevention and control of the disease.[Method]The lungs from dead sheep were aseptically taken,and laboratory diagnosis was conducted based on clinical symptoms.Pathogenic bacteria were isolated and cultured.Morphological observation,biochemical test,16S rRNA PCR amplification and sequencing,kmt Ⅰgene identification,capsule serotyping,virulence gene detection,drug susceptibility test,drug resistance gene detection and mouse pathogenicity test were used to identify and analyze the pathogenic characteristics of the isolated bacteria.[Result]The pathogenic strain was Pasteurella multocida.The isolated bacteria showed round raised colonies with smooth,grayish white surface on the blood plate,and Gram staining showed that the Gram-negative brevibacterium was clustered into lines or scattered in single.Biochemical test results showed that the strain could ferment maltose,arabinose,sucrose,xylose,glucose,fructose,trehalose,mannitol,urease,sorbitol and and ONPG,and arginine acid hydrolysis and indigo test were positive.Serotyping of the capsule showed that the isolate was Pasteurella multocida capsular serotype D.The drug sensitivity test showed that the isolates were resistant to enrofloxacin,bacitracin and lomefloxacin,intermediary to 4 drugs such as neomycin,and sensitive to 16 drugs such as ampicillin.The isolates carried 14 virulence genes and 6 resistance genes.All mice died after infection with the isolated bacteria.[Conclusion]One Pasteurella multocida serotype D strain was successfully isolated,carrying a variety of virulence genes and drug resistance genes,and was pathogenic to mice.This study provided data for exploring the biological information and etiological characteristics of the sheep origin Pasteurella multocida.

张蕾;张备;董佳强;黄宣凯;李旭业;江波涛;王洪宝;史同瑞;陈亮;金振华;冯万宇;苗艳;兰世捷;秦平伟;沈思思;李丹

黑龙江省农业科学院畜牧兽医分院,齐齐哈尔 161000

畜牧业

多杀性巴氏杆菌分离鉴定毒力基因耐药性致病性

Pasteurella multocidaisolation and identificationvirulence genedrug resistancepathogenicity

《中国畜牧兽医》 2024 (001)

347-356 / 10

黑龙江省农业科学院畜牧兽医分院自拟课题(ZNKT-202212);齐齐哈尔市科技计划创新激励项目(CNYGG-2023019);黑龙江省农业科技创新跨越工程农业关键技术科技创新重点攻关项目"草食家畜新品种选育推广与产业应用"(CX23GG07)

10.16431/j.cnki.1671-7236.2024.01.035

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