Eicosapentaenoic acid代谢产物促进胰岛α细胞转分化为胰岛β细胞的作用研究OACSTPCD

Aim To investigate the role of metabolites of eicosapentaenoic acid(EPA)in promoting the transdifferentiation of pancreatic α cells to β cells.Methods Male C57BL/6J mice were injected intrap-eritoneally with 60 mg/kg streptozocin(STZ)for five consecutive days to establish a type 1 diabetes(T1DM)mouse model.After two weeks,they were randomly divided into model groups and 97％EPA diet intervention group,75％fish oil(50％EPA+25％DHA)diet intervention group,and random blood glu-cose was detected every week;after the model expired,the regeneration of pancreatic β cells in mouse pancre-as was observed by immunofluorescence staining.The islets of mice(obtained by crossing GCGicre mice and Rosa26tdTomato mice)specially marked with tdTomato flu-orescent protein was extracted,1 mmol·L-1 STZ was added to induce apoptosis of β cells,EPA metabolites were addded,cultured for 48 hours,and fluorescent staining was used to observe insulin and tdTomato ex-pression of fluorescence.EPA metabolites were added to the medium of mouse islet α cell line αTC1,and af-ter 48 hours,the expressions of insulin and glucagon were observed by fluorescent staining.Results Both EPA and fish oil dietary intervention could significantly reduce blood glucose levels in T1DM mice,improve glucose homeostasis,and co-localize cells of insulin and glucagon in pancreas.Co-localization of tdTomato fluorescent protein and insulin occurred.EPA metabo-lites could promote insulin secretion from pancreaticαTC1 cell line cells.Conclusion EPA and its me-tabolites can alleviate hyperglycemia in T1DM mice by promoting the transdifferentiation of pancreatic α cells into β cells,thus increasing insulin secretion.