中外葡萄与葡萄酒Issue(1)：1-9,9.DOI:10.13414/j.cnki.zwpp.2024.01.001
混合菌剂对葡萄白粉病发生及叶际微生物多样性的影响
Effects of Mixed Fungicides on Occurrence of Grapevine Powdery Mildew and Microbial Diversity in Grapevine Phyllosphere
摘要
Abstract
In order to clarify the effects of common fungicides and biocontrol agents on the occurrence of grapevine powdery mildew and grapevine foliar microbial diversity,and further predict the function of foliar microorganisms,random sampling method was used to investigate grapevine powdery mildew in the field,the differences of microbial community diversity between fungicide and biocontrol treatment were analyzed by Illumina high-throughput sequencing technology combined with bioinformatics,and the microbial function was predicted by PICRUSt2.The results showed that the application of mixed bacterial agents could significantly reduce the incidence of powdery mildew.Chao1 index and Shannon index of grapevine leaf bacterial community decreased by 50.78%and 48.57%,respectively;Chao1 index of foliar fungal community decreased by 42.80%,and Shannon index increased by 0.07.At the phylum level,the dominant bacteria phyla of pesticide treatment were Bacillota and Actinomycetota,and the dominant fungi phyla were Ascomycota and Basidiomycota.At the genus level,after pesticide treatment,the bacteria with significantly increased relative abundance were Bacillus,and the fungi with significantly increased abundance were Erysiphe and Aspergillus.In the prediction of grapevine leaf microbial community function,489 MetaCyc pathways were evaluated.Based on the screening of P<0.05 and Fold Change(FC)>3,and 49 related pathways were obtained,of which two were significantly up-regulated and 47 were significantly down regulated,which were mainly related to the functions of grapevine nuclear acid metabolism,photorespiration,chlorophyll and vitamin synthesis.关键词
葡萄/农药处理/微生物多样性/葡萄白粉病Key words
grapevine/pesticide treatment/microbial diversity/grape powdery mildew分类
农业科技引用本文复制引用
杜鸿燕,何涛,陈吉越,胡祥,史亮涛,刘海刚,杨顺林,杜飞..混合菌剂对葡萄白粉病发生及叶际微生物多样性的影响[J].中外葡萄与葡萄酒,2024,(1):1-9,9.基金项目
财政部和农业农村部:国家现代农业产业技术体系专项资金(CARS-29-23) （CARS-29-23）
