葡萄MYB转录因子基因VvMYB30的克隆及其耐盐性分析OA

MYB transcription factors play an important role in responding to various abiotic stresses.This study used'Shine Muscat'grape as the material to clone a salt stress responsive MYB transcription factor gene VvMYB30,and conducted research on the characteristics and functions of VvMYB30.The sequence analysis indicated that the full-length coding sequence of VvMYB30 was 984 bp and encoded 327 amino acids.Bioinformatics analysis showed that VvMYB30 contained a conserved R2R3 domain and had the highest homology with sweet orange CsMYB30 transcription factors(64.75％similarity)and belonged to subgroup Ⅰ.Subcellular localization and transcriptional activation analysis showed that VvMYB30 was localized in the nucleus and had transcriptional activation activity.qRT-PCR showed that VvMYB30 was induced by salt stress,and the expression level reached the highest at 24 h,which was 6.86 times higher than the control.The salt tolerance test showed that the seed germination rate and seedling survival rate of VvMYB30 transgenic Arabidopsis were significantly higher than the wild type under salt stress,it indicated that overexpression of VvMYB30 could enhance the salt tolerance of transgenic Arabidopsis.