中外葡萄与葡萄酒Issue(1)：20-27,8.DOI:10.13414/j.cnki.zwpp.2024.01.003
葡萄MYB转录因子基因VvMYB30的克隆及其耐盐性分析
Cloning and Salt Tolerance Analysis of VvMYB30 from Grape(V.vinifera)
摘要
Abstract
MYB transcription factors play an important role in responding to various abiotic stresses.This study used'Shine Muscat'grape as the material to clone a salt stress responsive MYB transcription factor gene VvMYB30,and conducted research on the characteristics and functions of VvMYB30.The sequence analysis indicated that the full-length coding sequence of VvMYB30 was 984 bp and encoded 327 amino acids.Bioinformatics analysis showed that VvMYB30 contained a conserved R2R3 domain and had the highest homology with sweet orange CsMYB30 transcription factors(64.75％similarity)and belonged to subgroup Ⅰ.Subcellular localization and transcriptional activation analysis showed that VvMYB30 was localized in the nucleus and had transcriptional activation activity.qRT-PCR showed that VvMYB30 was induced by salt stress,and the expression level reached the highest at 24 h,which was 6.86 times higher than the control.The salt tolerance test showed that the seed germination rate and seedling survival rate of VvMYB30 transgenic Arabidopsis were significantly higher than the wild type under salt stress,it indicated that overexpression of VvMYB30 could enhance the salt tolerance of transgenic Arabidopsis.关键词
阳光玫瑰/MYB转录因子/盐胁迫/功能分析Key words
Shine Muscat/MYB transcription factor/salt stress/functional analysis分类
农业科技引用本文复制引用
解振强,许桓瑜,黄金霞,蔡善亚,李刚,赵鹏程..葡萄MYB转录因子基因VvMYB30的克隆及其耐盐性分析[J].中外葡萄与葡萄酒,2024,(1):20-27,8.基金项目
镇江市重点研发计划-现代农业(NY202002) （NY202002）
江苏农林职业技术学院科技项目(2020kj040)江苏省第六期"333高层次人才培养工程"第三层次培养对象资助项目 （2020kj040）
