多组学分析揭示三七镰刀菌属致病菌的生物学差异OA北大核心CSTPCD

Fusarium is one of the main pathogens of Panax notoginseng root-rot disease.In order to explore the molecular mechanism of biological differences among different species of Fusarium,the biological characteristics of Fusarium oxysporum,Fusarium proliferatum and Fusarium solani were analyzed by the multi-omics data.In this study,the strains were cultured with potato dextrose agar(PDA)medium,while spore germination,hyphal growth,spore yield and pathogenicity were determined.A wide range of targeted metabolomics and transcriptomics were used to perform high-throughput transcriptome sequencing and metabolite analysis on 9 samples of three Fusarium(F.oxysporium,F.proliferatum and F.solani).The differentially expressed genes(DEGs)and differential accumulated metabolites(DAMs)between Fusarium were compared and functional annotation and metabolic pathway analysis were combined with GO and KEGG databases.The results showed that the spore germination rate of F.solani was the fastest,followed by F.proliferatum,and F.oxysporium was the slowest.F.oxysporium had the fastest mycelium growth and the highest spore yield,followed by F.proliferatum and F.solani.The pathogenicity of F.oxysporium and F.solani to P.notoginseng was significantly higher than F.proliferatum.Analysis of DAMs showed that the amino acids were the major differential metabolites and differences in amino acid composition and content are the main factors causing the different growth of these three Fusarium species.The transcriptome data indicated that the DEGs functions between different Fusarium were mainly correlated with ribosome and oxidative phosphorylation.The results of this study provide a theoretical basis for the prevention and control of root-rot diseases of Panax notoginseng.