复合微生物菌剂的筛选及促生效果的研究OA

As a new type of green fertilizer with higher crop yield,better quality and more friendly and pollution-free environment,microbial fertilizer can not only rapidly increase the comprehensive fertility of soil surface in the field,but also significantly improve the climate and optimize the allocation of high-quality soil.As an important component of microbial fertilizer,microorganisms can be purified and prepared into microbial agents to promote plant growth.In order to screen out efficient microbial agents,through the pot experiment of promoting growth of a single strain on Fructus aurantii,the strains with obvious growth promoting effect were screened,and then the optimal strain combination was screened through the pot experiment of mandarin orange to explore the growth promoting effect of the combination on crops.Finally,the fertilizer efficiency experiment was conducted with corn,citrus and eucalyptus.A total of 25 strains of Fructus aurantii were screened,which could promote growth of Fructus aurantii seedlings.The 25 strains were randomly combined for the test,and the optimal 3 strains were selected,which were Micrococcus luteus(labeled as GXMD-hs-11052)and Bacillus subtilis(labeled as:GX-MD-hs-13035),Bacillus mycoides(labeled Gxun-30).The three strains were mixed to carry out growth promotion test.The test results showed that:compared with the control group,the plant height increased by 87.50％and the ground diameter increased by 52.48％.Compared with the control group,the plant height and ground diameter of eucalyptus seedlings increased by 0.33％and 9.16％when the fertilizer was added with the combined microbial agent.Compared with the control group,fresh weight and dry weight of corn increased by 1.25％and 1.25％.Compared with the control group,the ground diameter increased by 7.48％.A mixture of three strains was selected in the experiment,which had significant growth promotion effect on citrus,eucalyptus,maize and other crops,and provided the basis for the following large-scale application,which is of great significance for the development of green friendly fertilizer.