食品与机械2023，Vol.39Issue(12)：162-170,9.DOI:10.13652/j.spjx.1003.5788.2023.80693
酶解—膜分离耦合连续制备抗氧化性小分子透明质酸
Continuous preparation of antioxidant low molecular weight hyaluronic acid by coupling of enzymolysis and membrane separation
摘要
Abstract
Objective:This study aimed to construct an enzymolysis and membrane separation method to improve the production efficiency of low molecular hyaluronic acid(HA).Methods:A continuous enzymolysis and membrane separation coupling reaction system was designed,which consisted of an enzyme biological reaction tank and a flat polyethersulfone ultrafiltration membrane separation module.By evaluating membrane flux,membrane contamination,hyaluronic acid concentration,and yield,the effects of various factors on the separation of low molecular HA in the enzyme-membrane coupling system were systematically investigated.The process parameters of the enzyme-membrane coupling reaction were further optimized through orthogonal experiments.Results:The optimal process parameters for the preparation of low molecular hyaluronic acid by enzyme-membrane coupling reaction system were a stirring speed of 200 r/min,transmembrane pressure of 0.15 mPa,enzymolysis time of 4.0 h,and enzyme dosage of 5 g/100 g.In this method,two low molecular hyaluronic acids with different molecular weights(Mr)can be quickly prepared and separated,the low molecular weight hyaluronic acid(LMW-HA)with Mw between 10 000～50 000 and the HA oligosaccharides(O-HA)with Mr＜3 000 can be prepared rapidly by one-step approach.Both of these different molecular weights hyaluronic acid could scavenge DPPH and hydroxyl free radicals.Conclusion:The enzymolysis and membrane separation coupling method provides an efficient method for simultaneous and continuous production of LMW-HA and O-HA with different molecular weights and certain antioxidant properties.关键词
酶膜耦合反应/透明质酸/工艺优化/抗氧化/连续制备Key words
enzyme membrane reactors/hyaluronic acid/process optimization/antioxidation/continuous preparation引用本文复制引用
滕薇,刘俊辉,吴金鸿,刘树滔..酶解—膜分离耦合连续制备抗氧化性小分子透明质酸[J].食品与机械,2023,39(12):162-170,9.基金项目
国家十三五重点研发计划项目(编号:2019YFD0901902) （编号:2019YFD0901902）
国家自然科学基金项目(编号:31972017) （编号:31972017）
