食品与机械2024，Vol.40Issue(1)：47-54,8.DOI:10.13652/j.spjx.1003.5788.2023.80260
基于rGO-AuNPS修饰丝网印刷电极的电化学生物传感器快速检测甲基对硫磷
Rapid detection of methyl parathion by electrochemical biosensor based on rGO-AuNPS modified screen printing electrode
摘要
Abstract
Objective:In order to achieve rapid detection of methyl parathion.Methods:An acetylcholinesterase sensor was prepared based on gold nanoparticles and reduced graphene oxide for quantitative detection of Methyl parathion.Reduced graphene oxide,gold nanoparticles and acetylcholinesterase were successively modified on the surface of the screen printing electrode by layer-by-layer assembly method.The catalytic activity and impedance characteristics of the sensor,the relationship between the sensor's inhibition rate and MP concentration,and the actual sample detection were evaluated.Results:The prepared acetylcholinesterase biosensors showed excellent affinity for acetylthiocholine chloride with the Michaelis-Menten constant of 2.76 mmol/L.Under the optimal conditions,Methylparathion could be effectively detected with a linear range of 5 ng/mL to 500 ng/mL and a detection limit of 0.692 ng/mL.Conclusion:The method is simple,practical and stable.It provides a reliable method for rapid detection of organophosphorus pesticides.关键词
生物传感器/丝网印刷电极/甲基对硫磷/还原氧化石墨烯/纳米金Key words
biosensor/screen-printed electrode/methyl parathion/reduced graphene oxide/nano-gold引用本文复制引用
耿俊豪,李雪芝,周建平,陈昌华..基于rGO-AuNPS修饰丝网印刷电极的电化学生物传感器快速检测甲基对硫磷[J].食品与机械,2024,40(1):47-54,8.基金项目
新疆维吾尔自治区自然科学基金(编号:2021D01C102) （编号:2021D01C102）
新疆维吾尔自治区博士研究生科研创新项目(编号:XJ2021G048) （编号:XJ2021G048）
