食品与机械2024，Vol.40Issue(1)：55-62,67,9.DOI:10.13652/j.spjx.1003.5788.2023.80073
净化柱结合超高效液相色谱—串联质谱检测动物源性食品中16种喹诺酮药物
Establishment and application of clean-up combined with ultra-high performance liquid chromatography tandem mass spectrometry for the detection of 16 quinolones in animal-derived food
摘要
Abstract
Objective:A method for simultaneous detection of 16 quinolones in food of animal origin was developed byclean-up column pretreatment combined with ultra high performance liquid chromatography-tandem mass spectrometry.Methods:Food samples of animal origin were extracted using 80％acetonitrile(containing 0.2％formic acid).After purification on a Speedy Prep®-Quino 1 column,16 quinolone residues were detected by ultra-high performance liquid chromatography tandem mass spectrometry.Results:The results showed that the linear range of 16 quinolones was 1.6～40.0 μg/kg,the correlation coefficient r≥0.996 1,the limits of detection were 0.14～0.80 μg/kg,and the limits of quantification were 0.47～2.68 μg/kg.The recoveries of seven matrix after pretreatment were 62％～112％,and the relative standard deviations were 0.9％～18.7％.Conclusion:The method has the characteristics of fast detection speed and high sensitivity,and can be applied to animal derived food such as mutton,duck,beef,fish,eggs,pig kidney,duck skin.关键词
动物源性食品/喹诺酮/净化柱前处理/超高效液相色谱—串联质谱Key words
animal-derived food/quinolones/pretreatment of clean-up column/ultra-high performance liquid chromatography tandem mass spectrometry引用本文复制引用
余婷婷,梁松,黄文慧,何洁,严义勇..净化柱结合超高效液相色谱—串联质谱检测动物源性食品中16种喹诺酮药物[J].食品与机械,2024,40(1):55-62,67,9.基金项目
广东省粤港湾联合创新领域项目(编号:2021A0505080003) （编号:2021A0505080003）
资助深圳市技术攻关重点项目(编号:JSGG20191115141601721) （编号:JSGG20191115141601721）
