食品与机械2024，Vol.40Issue(1)：167-174,8.DOI:10.13652/j.spjx.1003.5788.2023.80428
葛根蛋白酶解物大孔树脂吸附工艺优化及其体外抗氧化活性研究
Optimization of macroporous resin adsorption process of enzymatic hydrolysis of Pueraria protein and its antioxidant activity in vitro by response surface method
摘要
Abstract
Objective:This study aims to optimize the adsorption of enzymatic hydrolysis of Pueraria protein by macroporous resin and maximize the antioxidant properties in vitro.Methods:The Box-Behnken response surface method was used to determine the optimization of adsorption of enzymatic hydrolysis of Pueraria protein.Using VC as a control,the antioxidant activity of Kase was determined before and after purification under the optimal purification process.Results:The optimal adsorption-desorption process of macroporous resin is:the mass concentration of the sample solution was 10.0 mg/mL,the flow rate of the eluent was 2.6 mL/min,and the volume fraction of ethanol in the eluent was 74％.After adsorption,the content of enzymatic hydrolysis of Pueraria protein increased to 37.19％.The scavenging rate of DPPH,ABTS+,and hydroxyl radicals of Pueraria Mirifica protease after adsorption was stronger than before adsorption.Conclusion:This study shows that enzymatic hydrolysis of Pueraria protein after adsorption of macroporous resin has good antioxidant effect,which can be used as a potential protein polypeptide in food,providing data reference for further research of enzymatic hydrolysis of Pueraria protein.关键词
葛根蛋白/酶解物/大孔树脂/抗氧化活性/吸附Key words
Pueraria protein/enzymatic hydrolysis/macroporous resins/antioxidant activity/adsorption引用本文复制引用
岳奕含,严铭铭,邵帅,丁奕元,江婷..葛根蛋白酶解物大孔树脂吸附工艺优化及其体外抗氧化活性研究[J].食品与机械,2024,40(1):167-174,8.基金项目
吉林省科技发展计划项目(编号:20210401111YY) （编号:20210401111YY）
吉林省发改委产业技术研究与开发项目(编号:2023C027-4) （编号:2023C027-4）
吉林省教育厅人文社科研究项目(编号:JJKH20211006SK) （编号:JJKH20211006SK）
