蛋鸡脂肪肝出血综合征对肝脏及腹脂转录组的影响研究OACSTPCD

This study was to identify and compare the transcriptomic profiles of liver and abdominal fat tissue of laying hens with fatty liver hemorrhage syndrome(FLHS),to reveal the important interaction between liver and abdominal fat,key genes and the underlying mecha-nisms,and to provide theoretical basis for precise targeting of liver and abdominal fat drugs.The FLHS model of laying hens was constructed by high-energy low-protein diet.Then,the gene expression profiles of liver and abdominal fat of FLHS laying hens and normal laying hens were compared by RNA-Seq.GO annotation,KEGG and GSEA pathway enrichment analysis were used to elucidate the critical biological processes and signaling pathways in FLHS pathogenesis enriched by differential genes of liver and abdominal fat from FLHS laying hens.Fi-nally,RT-PCR and RNA-Seq data were employed to verify the differential expressions of the key genes.The results showed that high-ener-gy low-protein diet successfully triggered FLHS in the laying hens with significant alterations of transcription profiles of liver and abdominal fat.By our RNA-seq data,we screened out 443 differential genes(151 upregulated,292 downregulated)and 526 differential genes(409 upregulated,117 downregulated)in liver and abdomen fat,respectively,of FLHS laying hens,as compared with the controls(| log2 FC|≥1,P＜0.05).The functionalenrichmentanalysissuggestedthatthedifferentialgenesidentifiedintheliver,including PCK1and APOA1,had a higher enrichment in the metabolic process and the PPAR signaling pathway,accelerating liver lipid deposition.However,IL-6,SOCS3 and other differential genes identified in the abdominal fat exhibited a tendency to be predominantly enriched in the regulation of the immune process and the JAK-STAT signaling pathway,contributing to inflammation.Liver and abdominal fat were both involved and had a critical interaction in the development of FLHS in the laying hens through different pathways.Specific targeted interference of key differential genes and signaling pathways between liver and abdominal fat was of great significance and should be considered to apply to optimizing the prevention and treatment of FLHS.