牛羊毛发中3种β-受体激动剂药物残留测定的EMR-Lipid方法研究OACSTPCD

A UPLC-MS/MS method was developed using the enhanced matrix removal technology(EMR-Lipid)for rapid determination of residues of β-receptor agonist drugs(clenbuterol,ractopamine,salbutamol)in cattle and sheep hair.The samples to be tested were pre-pared by selecting,cleaning,drying and grinding.After acid hydrolysis and ammonified acetonitrile extraction,the hair samples were poured into the Captiva EMR-Lipid purification columns.The ultra-high performance liquid chromatography-tandem mass spectrometry instrument equipped with an ESI+electric spray ion source was used for determination under a multi-reaction monitoring mode(MRM),and the internal standard method was applied for quantitative analysis.The results showed that the linear range of the drug had a good linear correlation in the concentration range of 0.1-100 ng/mL,and the correlation coefficient was≥0.995.The limit of detection(LOD)of this method was 0.2 μg/kg,and its limit of quantification(LOQ)was 0.5 μg/kg.The average recovery rate of the drugs was 70%-120%,and its relative stand-ard deviation was≥20%at concentrations of 1,20,100 μg/kg of the drugs.To sum up,the present method was convenient for sampling,with less pre-treatment steps,high sensitivity and precision;which would meet the residue detection requirements and might serve as refer-ence for determination of residues of β-receptor agonists in animal products.