畜牧与兽医2024，Vol.56Issue(2)：55-61,7.
牛羊毛发中3种β-受体激动剂药物残留测定的EMR-Lipid方法研究
Method for determination of doping drug desidues with cattle and sheep hair as target tissues based on the EMR-Lipid technology
摘要
Abstract
A UPLC-MS/MS method was developed using the enhanced matrix removal technology(EMR-Lipid)for rapid determination of residues of β-receptor agonist drugs(clenbuterol,ractopamine,salbutamol)in cattle and sheep hair.The samples to be tested were pre-pared by selecting,cleaning,drying and grinding.After acid hydrolysis and ammonified acetonitrile extraction,the hair samples were poured into the Captiva EMR-Lipid purification columns.The ultra-high performance liquid chromatography-tandem mass spectrometry instrument equipped with an ESI+electric spray ion source was used for determination under a multi-reaction monitoring mode(MRM),and the internal standard method was applied for quantitative analysis.The results showed that the linear range of the drug had a good linear correlation in the concentration range of 0.1-100 ng/mL,and the correlation coefficient was≥0.995.The limit of detection(LOD)of this method was 0.2 μg/kg,and its limit of quantification(LOQ)was 0.5 μg/kg.The average recovery rate of the drugs was 70%-120%,and its relative stand-ard deviation was≥20%at concentrations of 1,20,100 μg/kg of the drugs.To sum up,the present method was convenient for sampling,with less pre-treatment steps,high sensitivity and precision;which would meet the residue detection requirements and might serve as refer-ence for determination of residues of β-receptor agonists in animal products.关键词
牛羊毛发/β-受体激动剂/EMR-Lipid技术/残留检测Key words
cattle and sheep hair/β-receptor agonists/EMR-Lipid technology/residue detection分类
农业科技引用本文复制引用
李文辉,孙志文,于寒冰,倪香艳,张晶晶,魏紫嫣,王乐宜,赵雅妮,赵莹,方芳..牛羊毛发中3种β-受体激动剂药物残留测定的EMR-Lipid方法研究[J].畜牧与兽医,2024,56(2):55-61,7.基金项目
北京市农业科技项目(20170311) （20170311）
北京市农业农村局配套项目(202101) （202101）
