2例犬口腔肉瘤的病理学分析OA

Oral sarcomas are common oral tumors in dogs,particularly in geriatric dogs.In this study,two cases of oral masses in dogs were subjected to histopathological analysis using hematoxylin and eosin(H.E.)staining and immunohistochemistry detection.H.E.staining revealed proliferating spindle cells and local melanin granules in the tumor tissues of both dogs.Secondary hemorrhage and necrotic cystic changes were observed.Under high magnification,cells exhibited pleomorphism,atypia,prominent nucleoli,and frequent mitosis,leading to a preliminary diagnosis of malignant melanoma or soft tissue sarcoma.Immunohistochemical results showed negative expression of broad-spectrum cytokeratin(CKp)and positive expression of vimentin in both cases,epithelial membrane antigen(EMA)in case 1 and human cytokeratin antigen cluster(Cam5.2)in case 2 were negatively expressed,indicating an interstitial origin of the tumor.Specific antibodies such as Melan-A,S-100,and human melanoma black-45(HMB45)were all negative,reducing the possibility of melanoma and nerve sheath sarcoma.In case 1,α-smooth muscle actin(SMA)and Desmin were partially positive in some areas,suggesting partial myogenic differentiation.Case 2 showed no expression of myogenic markers.In conclusion,both cases of oral masses in dogs were diagnosed as high-grade soft tissue sarcomas.