基于UPLC特征图谱及两种成分含量测定的青翘质量评价OA

Objective:To establish UPLC characteristic chromatograms and determine the contents of two active components of Fructus Forsythia for providing basis for the quality evaluation of Fructus Forsythia.Methods:UPLC method was used to establish the characteristic atlas of Fructus For-sythia,and the quality of 12 batches of Fructus Forsythia from different habitats and different processing methods was analyzed by similarity evalua-tion,combined with cluster analysis(CA),principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA).The contents of forsythin and forsythoside A were determined according to the determination of Fructus Forsythia content in the 2020 edition of Chinese Pharmacopoeia.Results:There were 7 common peaks in UPLC characteristic chromatogram of Fructus Forsythia,and the similarity was greater than 0.98;The 12 batches of Fructus Forsythia were basically classified into 4 groups by CA and PCA,and there was no obvious regularity in origin and processing method;Three quality difference markers were determined by OPLS-DA analysis,namely,chromatographic peak 5(forsytho-side A),peak 2 and peak 4.The content determination results showed that the contents of forsythin and forsythoside A in the samples from Henan and Shanxi production areas were in line with the provisions of the Chinese Pharmacopoeia,and the contents of forsythin and forsythoside A in the samples from Xixia in Henan province were significantly different.Conclusion:This method can be used to evaluate the quality difference of the samples from different places of origin,and provide an experimental basis for the comprehensive utilization of Fructus Forsythia.