分析化学2024，Vol.52Issue(1)：54-61,8.DOI:10.19756/j.issn.0253-3820.231453
利用冷冻扫描电子显微镜观察金纳米颗粒原位标记红细胞膜阴离子通道蛋白
In Situ Labeling of Erythrocyte Membrane Anion Channel Proteins with Gold Nanoparticles Observed by Cryo-Scanning Electron Microscopy
摘要
Abstract
Band 3 protein is an important channel protein in the erythrocyte membrane which mediates the anion transport process inside and outside the cell membrane,as well as contributes to the maintenance of erythrocyte morphology,and has important physiological functions.However,the distribution state of this protein in the primary cell membrane is not known.Cryo-scanning electron microscopy enables imaging of the surface morphology of biological samples in a near-physiological state.In order to investigate the distribution of band 3 protein on erythrocyte membranes under physiological conditions,the present study utilized 5-nm gold nanoparticles modified with the antibodies to specifically bind to the band 3 protein on human blood erythrocyte membranes and imaged them by cryo-scanning electron microscopy,to obtain distribution of band 3 protein on human blood erythrocyte membranes.The results showed that the membrane proteins on the erythrocyte membranes tended to be clustered and distributed to form ″protein islands″,and band 3 proteins were mainly distributed in these protein islands,which were tightly connected with each other to form several functional microregions to play their respective roles.关键词
带3蛋白/冷冻扫描电子显微镜/金纳米颗粒/原位标记/蛋白分布/蛋白岛Key words
Band 3 protein/Cryo-scanning electron microscopy/Gold nanoparticles/In situ labeling/Protein distribution/Protein islands引用本文复制引用
程思航,王慧利,于洋,张金瑞,王宏达..利用冷冻扫描电子显微镜观察金纳米颗粒原位标记红细胞膜阴离子通道蛋白[J].分析化学,2024,52(1):54-61,8.基金项目
国家自然科学基金项目(Nos.21727816,21721003,22150003)和中国科学院科学仪器研制项目(No.ZDKYYQ20220005)资助. Supported by the National Natural Science Foundation of China(Nos.21727816,21721003,22150003)and the Scientific Instrument Developing Project of Chinese Academy of Sciences(No.ZDKYYQ20220005). （Nos.21727816,21721003,22150003）
