分析化学2024，Vol.52Issue(1)：93-101,9.DOI:10.19756/j.issn.0253-3820.231156
3D打印制备复杂截面惯性流道及微粒惯性聚焦特性研究
Three-dimensional Printing of Inertial Microchannel with Complex Cross-section and Characterization of Particle Inertial Focusing
摘要
Abstract
Projection micro stereolithography three-dimensional(3D)printing method was proposed in this study to fabricate complex microchannels of combined cross-sections.By using 3D printing and polydimethylsiloxane(PDMS)replication methods,two inertial microfluidic chips of three-step and five-step cross-sections were fabricated,and the dimension precisions of the microchannels were controlled within 20 μm.Using the microfluidic chips,the movements of two fluorescent polystyrene particles with diameters of 10 and 6 μm in the stepped channels were investigated.In addition,numerical simulations were applied to demonstrate the inertial focusing mechanisms of particles in the channels.It was found that 10-μm particles had three equilibrium positions in the three-step channel,which located at the inner walls of the three steps,respectively,and most particles focused at the inner step.The 6-μm particles also had three equilibrium positions in the three-step channel.However,the particles migrated to the middle and the outer steps under high flow rates.In the five-step channel,when the flow rate was increased gradually,10-μm particles had a single and two equilibrium positions,respectively,and the particles migrated towards the inner channel wall under high flow rates.In comparison to 10-μm particles,6-μm particles had two stable equilibrium positions in the five-step channel at all flow rate range.It could be concluded that the quantity,shape and strength of the secondary flow vortex could be altered by changing structure of the combined cross-section,thus the equilibrium positions and quantities of the focusing particles could be also regulated.The research outcome might provide new insights for precise cell inertial manipulation and promote the application and development of inertial microfluidic technology in biomedical and other fields.关键词
微流控/3D打印/复杂截面/惯性聚焦/二次流调控Key words
Microfluidics/Three-dimensional printing/Complex cross-section/Inertial focusing/Secondary flow regulation引用本文复制引用
顾乔,张鑫杰,刘尧,包洋,朱行杰,陈亚伟..3D打印制备复杂截面惯性流道及微粒惯性聚焦特性研究[J].分析化学,2024,52(1):93-101,9.基金项目
国家自然科学基金项目(No.51905150)、中央高校基本科研业务费专项资金项目(No.B220202024)、常州市卫健委青年人才科技项目(No.QN202115)和常州市科技计划项目(No.CE20225046)资助. Supported by the National Natural Science Foundation of China(No.51905150),the Fundamental Research Funds for the Central Universities(No.B220202024),the Changzhou Health Commission Youth Science and Technology Project(No.QN202115),and the Changzhou Science and Technology Bureau Program(No.CE20225046). （No.51905150）
