华东理工大学学报（自然科学版）2024，Vol.50Issue(1)：54-61,8.DOI:10.14135/j.cnki.1006-3080.20221109003
甘蔗糖蜜促进类球红细菌辅酶Q10的糖转化效率
Cane Molasses Promotes the Sugar Conversion Efficiency of Coenzyme Q10 in Rhodobacter sphaeroides
摘要
Abstract
The influence of cane molasses on the biosynthesis of coenzyme Q10 produced by Rhodobacter sphaeroides was investigated.The cane molasses adding process was optimized,which improved the sugar conversion rate and greatly reduced the production cost.The results showed that different carbon source substrates had a great influence on the synthesis of coenzyme Q10 products,and glucose was the most suitable carbon source,which was beneficial to the growth of Rhodobacter sphaeroides and the synthesis of coenzyme Q10.Through orthogonal experiments,the fermentation medium of coenzyme Q10 was optimized.When the mass concentrations of sugarcane molasses,glucose and KH2PO4 were 20 g/L,30 g/L and 1 g/L,respectively,the growth and coenzyme Q10 synthesis of erythroid bacteria were favorable,and the fermentation titer of coenzyme Q10 reached 380.1 mg/L.The sugar conversion rate was 12.71 mg/g.The fermentation was amplified on a 30 L fermenter,and the physiological parameters of coenzyme Q10 fermentation were collected and analyzed,which further verified that sugarcane molasses was conducive to the maintenance of the metabolic activity of thalli,and thus promoted the biosynthesis of coenzyme Q10.关键词
类球红细菌/甘蔗糖蜜/辅酶Q10/发酵优化/糖转化效率Key words
Rhodobacter sphaeroides/cane molasses/coenzyme Q10/fermentation optimization/sugar conversion rate分类
生物科学引用本文复制引用
葛燕,栗波,刘爱军,肖慈英,陈必钦,朱志春,梁剑光,庄英萍,王泽建..甘蔗糖蜜促进类球红细菌辅酶Q10的糖转化效率[J].华东理工大学学报（自然科学版）,2024,50(1):54-61,8.基金项目
江苏省研究生科研与实践创新计划项目(KYCX21_2866) （KYCX21_2866）
