水稻DUF1771基因家族的全基因组鉴定与表达谱分析OA北大核心CSTPCD

The domain of unknown function 1771(DUF1771)gene family in plants has not been described previously.This research identified 5 members of DUF1771 gene family in Oryza sativa through bioinformatics analysis,then the phylogenetic tree analysis was conducted with these 5 members and 4 members of DUF1771 gene family identified in Arabidopsis thaliana.All 9 members were divided into 2 subfamilies.Conserved motif analysis revealed that motif 1 and motif 2 were present in all DUF1771 members.The promoters of all 5 OsDUF1771 gene family members had relatively few cis-acting elements related to various stress responses and hormone responses,and OsDUF1771-1 had the least.Real-time fluorescence quantitative PCR analysis displayed that the expression levels of OsDUF1771 family members in rice before and after various plant hormone treatments showed little variation overall.When rice was treated with low temperature,drought,and Magnaporthe oryzae,the expression levels of OsDUF1771-1,2,3,5 showed some changes,but the changing ranges were small.OsDUF1771-4 had the largest expression change to low temperature treatment,and the expression level was 4.54 times that of the untreated sample at 12 h of treatment.The O2-site element related to seed storage protein metabolism regulation was found in the promoters of 4 OsDUF1771 family members,but not in the promoters of OsDUF1771-1,which was consistent with the tissue expression profile detection results.OsDUF1771-2,3,4,5 all had the highest expression levels in the panicles,while OsDUF1771-1 had the highest expression levels in the leave,and the encoded protein of OsDUF1771-1 was predicted to be located in the chloroplast.In addition,the promoter of OsDUF1771-3 contained the endosperm expression element GCN4-motif,further indicated that OsDUF1771-3 played a role in the metabolism of endosperm storage proteins in rice seeds.This study provides important clues for in-depth analysis of the biological functions of OsDUF1771 family members.