上海海洋大学学报2024，Vol.33Issue(1)：43-52,10.DOI:10.12024/jsou.20230204071
中华绒螯蟹3种亲本规格子代的生长、肠道微生物菌群和代谢组学分析
Studies on growth,metabolomics and intestinal microbes of offspring from the three parental standards of Eriocheir sinensis
摘要
Abstract
The parental sizes of Chinese mitten crab(Eriocheir sinensis)and the growth performance of its offspring have become interestingly main topics in aquaculture industry in recent years.In this study,we applied individual growth comparison and metabolomics techniques to investigate molting and growth characteristics,intestinal microbes and hepatopancreas metabolites compositions in offspring(Size A,Size B and Size C)of the three parental standards of Chinese mitten crab(200-250 g for females and 300-350 g for males;250-300 g for females and 350-400 g for males;＞300 g for females and＞400 g for males).The results showed that the growth performance in the offspring of size C was better than the other two sizes in the two individual molting and growth measurements,but there were no significant differences in the molting weight gain rate,molting interval,fatness,hepatopancreas index and muscle index among the three parental sizes of offspring.The results of intestinal microbes analysis showed that α diversity index of the size A was lower than the other two sizes,but there was no significant differences among them,while the PCoA analysis showed significant differences of intestinal microbes composition among the three sizes at the genus level.Metabolomics analysis of hepatopanacas found a total of 100 different metabolites between the sizes A and B,77 different metabolites between the sizes B and C,and 79 different metabolites between the sizes A and C.Pearson correlation analysis between different intestinal microbes and metabolites revealed that the metabolite Biopterin was significantly positively correlated with Genus Paraclostridium in the size A;the the metabolite N-acetyl-d-mannosamine was significantly negatively correlated with Phylum Nitrospira and Genus Nitrospira in the size B;the metabolite tetrahydrodipyridinate carboxylate was negatively correlated with Genus Lactobacillus in the size C.The current results of this study indicated some differences have been detected in molting and growth,intestinal microbes and hepatopancreas metabolites composition among the offspring of different parental sizes in Chinese mitten crab.关键词
中华绒螯蟹/亲蟹大小/蜕壳生长/肠道菌群/代谢组学Key words
Eriocheir sinensis/parent size/molting and growth/intestinal microbes/metabonomics分类
农业科技引用本文复制引用
周振旗,关伟晔,谢昕洋,侯鑫,王军,王成辉..中华绒螯蟹3种亲本规格子代的生长、肠道微生物菌群和代谢组学分析[J].上海海洋大学学报,2024,33(1):43-52,10.基金项目
上海市中华绒螯蟹现代农业产业技术体系建设项目(沪农科产字[2022]第4号) （沪农科产字[2022]第4号）
