医药导报2024，Vol.43Issue(3)：345-351,7.DOI:10.3870/j.issn.1004-0781.2024.03.003
虫草素及其代谢物3′-脱氧肌苷在大鼠体内的药动学
Pharmacokinetics of Cordycepin and Its Metabolite 3′-Deoxyinosine in Rats
摘要
Abstract
Objective To establish a method of LC-MS/MS for determining cordycepin(Cor)and 3′-deoxyinosine(3′-Deo)concentration in rat plasma,and to study their pharmacokinetics in rats.Methods Protein was precipitated with methanol using 2-chloadenosine(2-Chl)as an internal standard.The chromatography was performed on Kinetex C18(3 mm×100 mm,2.6 μm,Phenomenex,USA)with gradient elution in aqueous(5 mmol·L-1 ammonium acetate)-methanol solution as mobile phase.ESI ion source was used for mass spectrometry,and positive ion multiple reaction monitoring(MRM)was used for scanning detection.The pharmacokinetics of Cor and 3′-Deo after oral administration of Cor(10 mg·kg-1)were studied in rats.Results Cor at 0.5-100 ng·mL-1 and 3′-Deo at 1-200 ng·mL-1 had good linearity,and the lower limits of quantification were 0.5 and 1 ng·mL-1,respectively.After oral administration of Cor in rats,the plasma concentration of Cor was low,which was mainly converted into the metabolite 3′-Deo.The Cmax of Cor and 3′-Deo were(5.4±3.4)and(142.0±50.0)ng·mL-1,and AUC0-360min min were(658.4±459.3)and(18 034.9±4 981.1)ng·min·mL-1,respectively.Conclusion The method is simple,sensi-tive,and accurate,which is suitable for determining Cor and 3′-Deo concentration in plasma and the pharmacokinetic study.关键词
虫草素/3′-脱氧肌苷/药动学/液相色谱-串联质谱法Key words
Cordycepin/3′-deoxyinosine/Pharmacokinetics/Liquid chromatography tandem mass spectrometry分类
医药卫生引用本文复制引用
胡楠,蒋振伟,钱敏燕,张雯婷,陈陆俊,郑晓,应汉杰,蒋敬庭..虫草素及其代谢物3′-脱氧肌苷在大鼠体内的药动学[J].医药导报,2024,43(3):345-351,7.基金项目
国家自然科学基金资助项目(32270955,81972869) （32270955,81972869）
江苏省中医药科技发展计划(ZT202115) （ZT202115）
江苏省科技计划专项资金(重点研发计划社会发展)项目(BE2022719) （重点研发计划社会发展）
常州市社会发展科技支撑资金项目(CE20215030) （CE20215030）
常州市科技基础设施建设计划-常州市临床药学重点实验室(CM20223005). （CM20223005）
