中国病理生理杂志2024，Vol.40Issue(2)：255-264,10.DOI:10.3969/j.issn.1000-4718.2024.02.008
双氢青蒿素通过激活氯通道促进鼻咽癌CNE-2Z细胞放疗敏感性
Dihydroartemisinin promotes radiotherapy sensitivity of nasopharyngeal carcinoma CNE-2Z cells by activating chloride channels
摘要
Abstract
AIM:To investigate the role of ClC-3 chloride channel in the promotion of radio sensitization of na-sopharyngeal carcinoma CNE-2Z cells by dihydroartemisinin(DHA).METHODS:MTT was used to detect the inhibito-ry effect of DHA on the viability of CNE-2Z cells and normal nasopharyngeal epithelial NP69-SV40T cells,the radio sensi-tization effect of DHA on CNE-2Z cells was detected by cloning assay,the expression of ClC-3 protein was detected by Western blot,the expression of ClC-3 protein was down-regulated by siRNA technology,and the chlorine current of cells was recorded by whole cell patch-clamp technology.RESULTS:(1)Compared with NP69-SV40T cells,DHA selective-ly inhibited the proliferation of CNE-2Z cells,with IC10 values of(13.020±4.831)μmol/L and(5.244±1.050)μmol/L,respectively(P＜0.01).(2)The results of clonal formation experiments showed that DHA had a radio sensitizing effect on CNE-2Z cells,with a radio sensitization ratio of 1.9.(3)DHA could activate the chlorine channel of CNE-2Z cells and produce an outward chlorine current,but had no effect on the chlorine channel of NP69-SV40T cells.(4)DHA promoted the expression of ClC-3 chloric channel protein in CNE-2Z cells(P＜0.01).(5)Chlorine channel blocker NPPB could in-hibit the radio sensitizing effect of DHA on CNE-2Z cells by 1.84 times,and also inhibited the chlorine current activated by DHA.(6)the down-regulation of CNE-2Z ClC-3 protein could inhibit the radio sensitization effect of DHA on CNE-2Z cells by 4.19 times,and the activation of chlorine current by DHA on CNE-2Z cells was no longer produced.CONCLU-SION:DHA has a radio sensitizing effect on nasopharyngeal carcinoma CNE-2Z cells,which is likely to be related to the activation of ClC-3 chloride channel.关键词
鼻咽癌/双氢青蒿素/放射增敏/ClC-3氯通道Key words
nasopharyngeal carcinoma/dihydroartemisinin/radiation sensitization/ClC-3 chloride channels分类
医药卫生引用本文复制引用
刘世情,周丛然,唐鑫伟,周汉芬,李雪苛,侯秀颖,杨海峰,朱林燕..双氢青蒿素通过激活氯通道促进鼻咽癌CNE-2Z细胞放疗敏感性[J].中国病理生理杂志,2024,40(2):255-264,10.基金项目
国家自然科学基金资助项目(No.81872133) （No.81872133）
