中国食品添加剂2024，Vol.35Issue(2)：137-144,8.DOI:10.19804/j.issn1006-2513.2024.2.017
鹿茸菇多糖的提取工艺优化及其对大鼠运动性骨骼肌损伤影响的研究
Optimization of extraction of polysaccharides from pilose antler mushroom and its effect on exercise induced skeletal muscle injury in rats
摘要
Abstract
Response surface methodology(RSM)was used to optimize the extraction of polysaccharide from pilose antler mushroom,and to study its effect on exercise induced skeletal muscle injury in rats.The effects of enzyme addition,enzyme hydrolysis temperature and enzyme hydrolysis time on the yield of pilose antler mushroom polysaccharide were investigated.The results showed that when the enzyme dosage was 2.1％,the enzyme hydrolysis temperature was 50.4℃,and the enzyme hydrolysis time was 98 minutes,and with the above adjusted process parameters were repeated for three tests,and the average yield of pilose antler mushroom polysaccharide was 9.24％,which was close to the predicted value.The exercise test of rats showed that,after gavage of pilose antler mushroom polysaccharide,the levels of SOD and GSH-Px in rats significantly increased,the content of MDA decreased,and the levels of LDH and CK significantly decreased,these indicated that pilose antler mushroom polysaccharide had a good antioxidant capacity,and could reduce the permeability of skeletal muscle cell membrane,thereby reducing the damage to skeletal muscle caused by free radicals produced after exhaustive exercise in rats,and improving the antioxidant capacity of rats.关键词
鹿茸菇多糖/纤维素酶/响应面法/骨骼肌Key words
pilose antler mushroom polysaccharide/cellulase/response surface method/skeletal muscle分类
轻工纺织引用本文复制引用
刘冰洋,刘雪菲,房磊..鹿茸菇多糖的提取工艺优化及其对大鼠运动性骨骼肌损伤影响的研究[J].中国食品添加剂,2024,35(2):137-144,8.基金项目
吉林省教育厅科学研究规划项目(编号JJKH20210426SK). （编号JJKH20210426SK）
