华北农学报2024，Vol.39Issue(1)：113-119,7.DOI:10.7668/hbnxb.20194526
干旱胁迫下2种内生真菌对烟草生理生化指标及NAC基因表达的影响
Effects of Two Endophytic Fungi on Physiological and Biochemical Indexes and NAC Gene Expression in Tobacco under Drought Stress
摘要
Abstract
To investigate the effects of Piriformospora indica combined with Arbuscular mycorrhizal fungi(AMF)on drought resistance of tobacco,Yunyan 87 was inoculated with sterile water(CC),P.indica(CP),AMF(PC),P.indica and AMF(PP),and the contents of proline(Pro),superoxide dismutase(SOD),peroxidase(POD),malondialdehyde(MDA)and the expression of drought-related genes NTNAC1 and NAC4 in tobacco leav-es were determined under stress in the form of natural drought.The results showed that P.indica and AMF could promote the growth of above-ground and underground parts of tobacco,and chlorosis of leaves and symptoms of their drought were slight.After 7 days of drought stress,the contents of Pro in tobacco leaves of CP,PC and PP groups were 1.39,1.59 and 1.78 times higher than those of CC in group,respectively.The activities of SOD and POD in tobacco leaves increased first and then decreased.The activities of SOD in CP,PC and PP groups were 1.15,1.22 and 1.33 times higher than those in CC group,POD activity was 1.33,1.46 and 1.85 times higher than those of CC group,respectively.The content of MDA in tobacco leaves was decreased by 21.98％,23.98％and 24.84％in CP,PC and PP groups respectively.The expression levels of NTNAC1 and NAC4 in tobacco leaves were up-regula-ted.The expression levels of NTNAC1 in CP,PC and PP groups were 3.37,3.88 and 5.07 times higher than those in CC group,the expression levels of NAC4 gene were 3.04,3.59 and 5.56 times higher than those of CC group,re-spectively.This study indicates that P.indica and AMF showed significant synergistic effects,which could signifi-cantly improve the drought resistance of tobacco.关键词
烟草/印度梨形孢/丛枝菌根真菌/抗旱性Key words
Tobacco/Piriformospora indica/Arbuscular mycorrhizal fungi/Drought resistance分类
农业科技引用本文复制引用
孙文秀,邵晨阳,陈妍妍,聂明皓,李震,曹毅,刘应保..干旱胁迫下2种内生真菌对烟草生理生化指标及NAC基因表达的影响[J].华北农学报,2024,39(1):113-119,7.基金项目
国家自然科学基金项目(31701573) （31701573）
中国烟草总公司科技项目(110202201004(JY-04)) （110202201004(JY-04）
贵州省烟草公司黔西南州公司科技项目(202104) （202104）
