中国实用神经疾病杂志2024，Vol.27Issue(3)：271-276,6.DOI:10.12083/SYSJ.230838
星状神经节离断对脊髓损伤大鼠中枢性疼痛行为及CREB SGK1 NF-κB蛋白表达的影响
Impacts of stellate ganglion neurotomy on central pain behavior and the expressions of CREB,SGK1 and NF-κB proteins in spinal cord injury rats
摘要
Abstract
Objective To investigate the impacts of stellate ganglion neurotomy on central pain behavior and the expressions of cAMP-response element binding protein(CREB),serum and glucocorticoid induced kinase1(SGK1)and nuclear factor-κ B(NF-κ B)proteins in spinal cord injury(SCI)rats.Methods SD rats were randomly separated into sham surgery group(Sham group),model group(Model group)and treatment group(Treat group),6 cases of each group.The Model group and Treat group constructed SCI models.The Sham group did not use an iron ball to hit the spinal cord,and the other steps were the same.The Treat group underwent stellate ganglion dissection after spinal cord injury.The BBB motor function score was applied to evaluate motor function,the mechanical pain threshold experiment and thermal pain threshold experiment were applied to measure the paw withdrawal threshold(PWT)and paw withdrawal latency(PWL).ELISA was applied to detect serum levels of interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)and prostaglandin E2(PGE2).HE staining was applied to detect pathological changes in spinal cord tissue.TUNEL staining was applied to detect cell apoptosis.Western blot was applied to detect the expressions of CREB,SGK1,and NF-κB p65 proteins.Results The spinal cord tissue structure of rats in Sham group was intact,with normal cell morphology,uniform distribution of nerve fibers,and regular walking.Compared with the Sham group,the spinal cord tissue and cell integrity of the Model group were severely damaged,forming a large number of cavities,disordered arrangement of nerve fibers,and visible infiltration of a large number of inflammatory cells,the BBB motor function score,PWT,and PWL values were obviously decreased(P<0.05),the levels of IL-1β,TNF-α,PGE2,cell apoptosis,and the levels of CREB,SGK1,NF-κB p65 protein were obviously increased(P<0.05).Compared with the Model group,the spinal cord tissue structure and morphology of the Treat group were obviously improved,with improved spinal cord tissue cavitation,reduced inflammatory cell infiltration,and a normal arrangement of nerve fibers,the BBB motor function score,PWT,and PWL values were obviously increased(P<0.05),the levels of IL-1β,TNF-α,PGE2,cell apoptosis,and CREB,SGK1,NF-κB p65 protein were obviously decreased(P<0.05).Conclusion Stellate ganglion neurotomy can alleviate central pain behavior in SCI rats and reduce the levels of CREB,SGK1,and NF-κB proteins.关键词
星状神经节离断/脊髓损伤/中枢性疼痛行为/环磷腺苷效应元件结合蛋白/血清和糖皮质激素调节蛋白激酶1/核因子-κB/大鼠Key words
Stellate ganglion neurotomy/Spinal cord injury/Central pain behavior/CREB/SGK1/NF-κB/Rats分类
医药卫生引用本文复制引用
邓莉丽,毛冬梅,廖泽青,姚旌..星状神经节离断对脊髓损伤大鼠中枢性疼痛行为及CREB SGK1 NF-κB蛋白表达的影响[J].中国实用神经疾病杂志,2024,27(3):271-276,6.基金项目
贵州省卫健委2023年科学技术基金项目(编号:gzwkj2023-184) （编号:gzwkj2023-184）
