热带作物学报2024，Vol.45Issue(2)：394-404,11.DOI:10.3969/j.issn.1000-2561.2024.02.019
生防枯草芽孢杆菌Czk1固体发酵工艺条件优化
Optimization of Solid-state Fermentation Conditions of Antagonistic Bacillus subtilis Czk1
摘要
Abstract
Bacillus subtilis Czk1 is a rhizosphere antagonist with a very broad-spectrum antagonistic,growth promotion and induction of plant resistance.In order to fully utilize organic nutrients in agricultural wastes and promote biological control of soil-borne plant diseases,Czk1 was used as the research object in this study,chicken manure as the base fer-tilizer,corn straw,mushroom bran and coconut bran compost were used as the raw materials,and the liquid-solid two-phase fermentation process was adopted.The total number of viable bacteria and the number of spores were deter-mined by the dilution plate colony counting method.The formula,process parameters and culture conditions of Czk1 solid fermentation materials were determined by the single factor and orthogonal experiment method.The results showed that the optimal conditions for solid fermentation of Czk1 are raw material was mushroom bran and chicken manure compost with the inoculated weight of Czk1 at 10% ,the added amount of soybean powder at 10% ,the water content at 40% ,the fermentation temperature at 37℃ and the turning once every 72 h.Under the conditions,the total number of viable bacteria in Czk1 strain could reach 8.63×108 CFU/g.The number of spores was 8.37×108 CFU/g.The best fermentation material was corn straw and chicken manure compost,Czk1 inoculation amount of 10% ,adding 5% soybean powder,40% water content,fermentation temperature of 37℃ ,turning once every 72 h,the total number of vi-able bacteria in Czk1 inoculation strain could reach 6.53×108 CFU/g.The number of spores was 5.97×108 CFU/g.The total number of viable bacteria and the number of spores of Czk1 strain could reach 8.70×108 CFU/g and 6.90×108 CFU/g,re-spectively,when 30% mushroom bran was added.The physical and chemical properties of four fermentation biological fertilizer raw materials were determined,and significant differences were observed in their organic matter content and carbon to nitrogen ratio.Among them,chicken manure had the highest pH,total phosphorus,and total potassium content,while the carbon to nitrogen ratio was the lowest.The total content of nitrogen,phosphorus,and potassium was higher than that of corn straw,coconut bran,and mushroom bran;Corn straw has the highest electrical conductivity and or-ganic matter content as the raw material;Coconut bran has the highest total nitrogen content,while mushroom bran has the highest carbon to nitrogen ratio.The seed germination index of bio-organic fertilizer with different combinations of chicken manure,mushroom bran,corn straw,and coconut bran is greater than 80% .Therefore,high quality bioorganic fertilizer can be prepared from agricultural waste by selecting suitable fermentation production conditions.B.subtilis Czk1 has good development and utilization value.关键词
枯草芽孢杆菌/固体发酵/发酵工艺优化/生物有机肥/芽孢产量Key words
Bacillus subtilis/solid-state fermentation/fermentation condition optimization/bio-organic fertilizer/spore production分类
化学化工引用本文复制引用
贺春萍,翟纯鑫,吴贺,梁艳琼,谭施北,吴伟怀,易克贤..生防枯草芽孢杆菌Czk1固体发酵工艺条件优化[J].热带作物学报,2024,45(2):394-404,11.基金项目
海南省院士创新平台科研专项(No.YSPTZX202016) （No.YSPTZX202016）
海南省自然科学基金项目(No.320RC692) （No.320RC692）
国家天然橡胶产业技术体系病害防控岗位项目(No.CARS-33-BC1). （No.CARS-33-BC1）
