食品工业科技2024，Vol.45Issue(6)：128-141,14.DOI:10.13386/j.issn1002-0306.2023040151
不同诱导物对白地霉中高级醇脱氢酶的影响
Effects of Different Inducers on Higher Alcohol Dehydrogenase from Galactomyces geotrichum
摘要
Abstract
The inducers of the chemical or physical factors could affect the gene expression and transcription of strain directly or indirectly.In order to explore the inductive effect of higher alcohol on Galactomyces geotrichum,the G.geotrichum S12,derived from the soil,was induced by five higher alcohols,including n-propanol,n-butanol,isobutanol,n-hexanol and isoamyl alcohol.The effects of induction dose and induction time for degradation activity of different higher alcohols by strain S12 and its transforming enzyme were studied.The results showed that the enzyme activity formed by strain S12 was higher when the inducers were chosen as n-hexanol and isobutanol.The optimum induction time was 6 h when n-propanol,n-butanol,isobutanol and n-hexanol were used as inducers.While the optimum induction time of strain S12 and enzyme induced by isoamyl alcohol was 12 h.When n-propanol and n-hexanol were used as inducers,the optimal concentration was 1.5 g/L.While the optimal concentrations of strain S12 and enzyme induced by n-butanol,isobutanol and isoamyl alcohol were 1.0,0.5 and 2.5 g/L,respectively.The results of native polyacryplamide gel electrophoresis(Native-PAGE)indicated that dehydrogenase formation,molecular weight about 223 kDa,was induced by five higher alcohols.In particular,the strain,induced by hexanol,had much higher capability in degrading five higher alcohols at the same time.The products of the above five higher alcohols after catalyzing by G.geotrichum S12 induced by hexanol were their corresponding acids and esters.关键词
白地霉/高级醇脱氢酶/诱导物/酶活性/诱导条件Key words
Galactomyces geotrichum/higher alcohol dehydrogenase/inducer/enzyme activity/induction conditions分类
轻工纺织引用本文复制引用
朱静,杨晓聪,陈亚蓝,刘海波,陈龙,郑雪珂,师俊玲..不同诱导物对白地霉中高级醇脱氢酶的影响[J].食品工业科技,2024,45(6):128-141,14.基金项目
河南省重点研发与推广专项(科技攻关)项目(212102110314) （科技攻关）
河南省高等学校青年骨干教师培养计划(2019GGJS264) （2019GGJS264）
河南省青年科学基金项目(212300410228) （212300410228）
河南省本科高校研究性教学改革研究与实践项目(2022SYJXLX119) （2022SYJXLX119）
信阳农林学院2021年校级教育教学改革研究与实践项目(2021XJGLX21) （2021XJGLX21）
信阳农林学院高水平科研孵化器建设基金项目(FCL202014) （FCL202014）
信阳农林学院2019年度学校青年基金(2019LG009). （2019LG009）
