食品工业科技2024，Vol.45Issue(6)：178-185,8.DOI:10.13386/j.issn1002-0306.2023040026
响应面法优化相思藤黄酮提取工艺及其体外抗氧化活性分析
Optimization of Extraction Process by Response Surface Method and Analysis of Antioxidant Activityin Vitroof Total Flavonoids from Abrus precatorius Linn
摘要
Abstract
The objective of this study was to optimize the extraction process of flavonoids from Abrus precatorius Linn,and explored its antioxidant activity.Ethanol volume fraction,solid-liquid ratio,ultrasonic energy and ultrasonic extracting time as variables,the yield of flavonoids as evaluation index,single factor tests and Box-Behnken method were used to optimize the extraction process.The scavenging ability on DPPH,ABTS+ free radicals,and the reducing power of flavonoids from Abrus precatorius Linn were also detected to estimate the antioxidant activities.Results showed that,when solid-liquid ratio reached 1:50,the optimum extraction process(0.696%,highest yield)could be achieved under condition of ethanol volume fraction 82%,ultrasonic energy 507 J and ultrasonic extracting time 42 min.Half effective concentration being EC500.028 and 0.009 mg/mL respectively.Abrus precatorius Linn flavonoids were able to scavenge DPPH and ABTS+ free radicals effectively,and its ability risen as mass concentration increases,meaning it is superior to Trolox.When flavonoid concentration was set at 5 mg/mL,the reducing power on iron ion reached 2.42 mmol/L,which was weaker than Trolox.To sum up,ultrasonic extraction technology can be effectively employed to the extraction of Abrus precatorius Linn flavonoids,resulting in better antioxidant activity.关键词
相思藤/黄酮/提取工艺/抗氧化活性Key words
Abrus precatorius Linn/flavonoids/extraction technology/antioxidant activity分类
轻工纺织引用本文复制引用
龚受基,覃媚,戴梓茹,蒋红明,郭德军..响应面法优化相思藤黄酮提取工艺及其体外抗氧化活性分析[J].食品工业科技,2024,45(6):178-185,8.基金项目
广西壮族自治区教育厅:广西高校中青年教师科研基础能力提升项目(2022KY0425) （2022KY0425）
钦州市科技攻关(202014820). （202014820）
