食品工业科技2024，Vol.45Issue(6)：280-288,9.DOI:10.13386/j.issn1002-0306.2023050175
QuEChERS-超高效液相色谱-串联质谱法测定动物源性食品中30种抗寄生虫类药物残留
Determination of 30 Kinds of Antiparasitic Drugs in Animal-derived Foods by QuEChERS-UPLC-MS/MS
摘要
Abstract
Objective:A method for determining the residues of 30 antiparasitic drugs in animal-derived food using ultra-high-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was established.Methods:The samples were extracted with acetonitrile and 1%ammonia acetic ether and purified by QuEChERS.After purification,Waters ACQUITY UPLCTM BEH C18 column was used for separation,and a gradient elution of 10 mmol/L ammonium formate(containing 0.1%formic acid)aqueous solution,acetonitrile:methanol(50:50,v:v)was performed as the mobile phase.Detection was carried out using electrospray ionization(ESI)in both positive and negative ion modes using multiple reaction monitoring(MRM).Matrix-matched external standard quantification was used.Results:Under the optimized conditions,the 30 antiparasitic drugs showed good linearity within their respective linear ranges,with coefficient of determination(r2)greater than 0.99.The recoveries ranged from 70.1%to 111%,and the relative standard deviations were between 0.10%and 9.1%(n=6).The method detection limit ranged from 0.001 to 0.3 μg/kg,and the method quantification limit ranged from 0.004 to 1 μg/kg.Conclusion:The method is sensitive,accurate,and exhibits good repeatability and stability,making it suitable for detecting various antiparasitic drug residues in animal-derived food.关键词
抗寄生虫药物/超高效液相色谱-串联质谱法/QuEChERS/动物源性食品/药物残留Key words
antiparasitic drugs/ultra-high-performance liquid chromatography-tandem mass spectrometry/QuEChERS/animal derived food/drug residues分类
轻工纺织引用本文复制引用
冯丽凤,黄芊,叶梦薇,黄永辉,黄燕,林浩学,梁敏,许晖..QuEChERS-超高效液相色谱-串联质谱法测定动物源性食品中30种抗寄生虫类药物残留[J].食品工业科技,2024,45(6):280-288,9.基金项目
福建省市场监督管理局科技项目(FJMS2020004) （FJMS2020004）
国家市场监督管理总局科技计划项目(2021MK054). （2021MK054）
