动物生物安全实验室废弃物分类高压灭菌程序探索OA

In order to explore reasonable autoclaving procedures for different types of wastes in animal bio-safety laboratories,and to identify the influence of sterilization time and pulsation parameters of pulsating vacuum sterilizers on sterilization effectiveness,autoclaving procedures for different types of wastes(animal carcasses,feces as well as protective clothing,tool containers and other general wastes)were studied taking the chemical indicator card and biological indicator as a standard.For the settings of procedures,some parameters were fixed,including sterilization temperature at 121℃,4 times of pulsations and upper emptying limit of 80 kPa,to explore the effective procedures by setting different sterilization time and pulsation limits.The results showed that the sterilization of the carcasses and feces of pigs,cows,sheep and other large animals was qualified when the sterilization time was 90 min and the lower emptying limit was-10 kPa;and that of general wastes,small animal carcasses and poultry feces was qualified when the lower emptying limit was-80 kPa and the sterilization time was 30,60 and 120 min,respectively.In conclusion,by adjusting the sterilization time and pulsation parameters,the sterilization effect could be more ideal when different types of wastes were classified and disposed.A reference was provided for disposal of wastes in animal bio-safety laboratories.