洛索洛芬钠含量测定方法学研究OA

Objective To establish a liquid chromatography method for the determination of loxoprofen sodium content and conduct methodological research on it.Methods Liquid chromatography was used,with methanol water glacial acetic acid triethylamine(600∶400∶1∶1)as the mobile phase,with equal elution.The column temperature was 35℃,the flow rate was 1.0 ml/min,and the detection wavelength was 222 nm.Results The concentration of loxoprofen sodium was linear when its concentrate range was from 30 to 78 μg/ml(r=0.999 99).The repeatability,intermediate precision,accuracy,and durability are meet the requirements.The detection limit is 0.012 μg/ml,and the quantitative limit is 0.048 μg/ml.Conclusion This method has strong applicability,good durability,and high sensitivity,and can be used for the determination of the content of loxoprofen sodium raw materials,dissolution,content uniformity,and content determination of loxoprofen sodium tablets and capsules.