云南烟草种质库种子的生活力监测OA北大核心CSTPCD

[Objective]This study aims to implement vitality monitoring of resources in the tobacco germplasm repository to ensure the safe preservation of stock resources.[Methods]Materials:tobacco seeds stored in Genebank from 1979 to 2008 in Yunnan Institute of Tobacco Agricultural Sciences were collected as research objects Seed viability monitoring was carried out in 1992,2005 and 2019,respectively,and the germination rate and germination potential were analyzed statistically.[Results]The annual decline in seed germination potential and germination rate is related to the length of preservation.The longer the preservation period,the higher the annual vitality decline.However,the rate of decline in seed vitality is not consistent for seeds deposited in different years.The higher the initial vitality of the seeds before preservation,the slower the decline in vitality.[Conclusion]In order to ensure the long-term safe preservation of tobacco seeds,field management should be strengthened during the tobacco planting process,and tobacco seeds should be harvested under favorable meteorological conditions to improve seed quality.It is recommended that the germination rate of tobacco germplasm entering the repository should be ≥90％,but it should not be less than 70％if conditions are limited.Seeds with vitality dropping to 70％should be promptly monitored and warned,and breeding updates should be arranged.Under the preservation conditions of ultra-dry tobacco seeds with a moisture content＜5.0％and room temperature,the first monitoring interval should not be longer than 10 years,and each subsequent monitoring interval should be shortened accordingly based on the decline in tobacco seed vitality.