改良56℃热放散法与冷冻复融放散法对ABO型新生儿溶血病的临床应用价值比较OACSTPCD

Objective To compare the positive rate and the cost of modified 56℃heat elution method and freeze-thaw elution method in detecting ABO hemolytic disease of fetus and newborn(ABO-HDFN),and to discuss the clinical application value of the two methods.Methods All neonatal/umbilical cord blood samples received in our hospital in March 2023 were selected for ABO-HDFN detection(direct anti-human globulin test,free antibody test,elution test),and the elution method was used of modified 56℃heat elution and freeze-thraw elution simultaneously,the results of the two elution methods were compared and analyzed by specific statistical methods.Results A total of 256 non-O blood type neonatal/umbilical cord blood samples were included for ABO-HDFN detection,of which 145 were type A,109 were type B and 2 were type AB.The positive rate of modified 56℃heat elution method was 69.92%,and the freeze-thraw method was 64.45%,there is no statistical difference between the two methods,either in the condition of different blood type,different aggregation intensity and different DAT.Only when free antibody was positive,the modified 56℃heat elution method was better than the freeze-thraw method.Conclusion There is no statistical difference between the two methods for the diagnostic value of ABO-HDFN in most situations.Each laboratory can choose the method suitable for the laboratory according to the emergency degree of clinical patients,the equipment,consumables configuration,staff experience and time cost of the laboratory.